UHPLC-LTQ-Orbitrap MS combined with spike-in method for plasma metabonomics analysis of acute myocardial ischemia rats and pretreatment effect of Danqi Tongmai tablet
文献类型：期刊论文
| 作者 | Yan, Bingpeng1,2; Deng, Yanping2; Hou, Jinjun2 ; Bi, Qirui1; Yang, Min2; Jiang, Baohong2; Liu, Xuan2; Wu, Wanying2; Guo, Dean1,2
|
| 刊名 | MOLECULAR BIOSYSTEMS
 |
| 出版日期 | 2015 |
| 卷号 | 11期号:2页码:486-496 |
| ISSN号 | 1742-206X |
| DOI | 10.1039/c4mb00529e |
| 文献子类 | Article |
| 英文摘要 | Undoubtedly, metabonomics can reveal the comprehensive efficacies of traditional Chinese medicine (TCM) formulae and its complex mechanism at the molecular biological level. In this study, an attempt was made to address the pretreatment effect of a TCM formula. In this case, as a critical point, we should first know how to really reflect the various endogenous metabolites in a disease status before a TCM formula is employed in a therapeutic procedure. Here, we explored an approach that combined high resolution LTQ-Orbitrap mass spectrometry with a spike-in method to characterize endogenous metabolites in acute myocardial ischemia (AMI) rats. As a result, 19 potential biomarkers in rat plasma were identified and 10 related disturbed pathways were perturbed in the early stages of AMI development. Subsequently, the metabonomics method was applied to investigate the pretreatment effect of the TCM formula named the Danqi Tongmai tablet (DQTM). The results revealed that the DQTM pretreatment could reduce the AMI injury and partially regulate the perturbed TCA cycle and amino and nucleotide metabolism, which were presumable related to energy metabolism and myocardial cells apoptosis/necrosis. In conclusion, UHPLC-LTQ-Orbitrap MS combined with a spike-in method were successfully applied to the metabonomics analysis of DQTM, which demonstrated that not only a comprehensive metabolic profile in the early stages of AMI development was achieved, but also that the underlying holistic efficacies were assessed and it was helpful to understand the possible mechanism of pretreatment with DQTM. |
| WOS关键词 | TRADITIONAL CHINESE MEDICINE ; MASS-SPECTROMETRY ; REPERFUSION INJURY ; CHROMATOGRAPHY ; INFARCTION ; HEART ; SERUM ; METABOLOMICS ; COMBINATION ; DATABASE |
| 资助项目 | National Science & Technology Major Project Key New Drug Creation and Manufacturing program of the People's Republic of China[2011ZX09307-002-03] ; Special Scientific Research in the Chinese Medicine Industry of State Administration of Traditional Chinese Medicine[201307002] ; Twelfth Five-Year National Science & Technology Support Program[2012BAI29B06] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000348211900016 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276768]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Wanying |
| 作者单位 | 1.China Pharmaceut Univ, Coll Tradit Chinese Med, Nanjing 210009, Jiangsu, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Bingpeng,Deng, Yanping,Hou, Jinjun,et al. UHPLC-LTQ-Orbitrap MS combined with spike-in method for plasma metabonomics analysis of acute myocardial ischemia rats and pretreatment effect of Danqi Tongmai tablet[J]. MOLECULAR BIOSYSTEMS,2015,11(2):486-496. |
| APA | Yan, Bingpeng.,Deng, Yanping.,Hou, Jinjun.,Bi, Qirui.,Yang, Min.,...&Guo, Dean.(2015).UHPLC-LTQ-Orbitrap MS combined with spike-in method for plasma metabonomics analysis of acute myocardial ischemia rats and pretreatment effect of Danqi Tongmai tablet.MOLECULAR BIOSYSTEMS,11(2),486-496. |
| MLA | Yan, Bingpeng,et al."UHPLC-LTQ-Orbitrap MS combined with spike-in method for plasma metabonomics analysis of acute myocardial ischemia rats and pretreatment effect of Danqi Tongmai tablet".MOLECULAR BIOSYSTEMS 11.2(2015):486-496. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。