Rapid characterization and determination of multiple components in Bu-Shen-Yi-Qi-Fang by high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry
文献类型：期刊论文
| 作者 | Nurahmat, Mammat1,2; Chen, Meixia2; Luo, Qingli2; Ling, Yun3; Dong, Jingcheng2; Huang, Chenggang3
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
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| 出版日期 | 2014-12 |
| 卷号 | 37期号:23页码:3509-3517 |
| 关键词 | Bu-Shen-Yi-Qi-Fang High-Performance Liquid Chromatography Mass Spectrometry Rapid Characterization Traditional Chinese Medicine |
| ISSN号 | 1615-9306 |
| DOI | 10.1002/jssc.201400673 |
| 文献子类 | Article |
| 英文摘要 | In this study, a qualitative and quantitative analysis using high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry was performed for the quality control of Bu-Shen-Yi-Qi-Fang, a traditional Chinese formula used for asthma. Thirty-four compounds, including flavonoids, isoflavonoids, triterpenoid saponins, and iridoid glycosides were identified or tentatively characterized by comparing their retention times and mass spectra with those of authentic standards or literature data. Sixteen components were considered as the main bioactive constituents of Bu-Shen-Yi-Qi-Fang and they were chosen as the chemical markers in quantitative analysis, including catalpol, leonuride, calycosin-7-O-beta-D-glucoside, hyperoside, acteoside, formononetin-7-O-beta-D-glucoside, epimedin A, calycosin, icariin, epimedin B, epimedin C, formononetin, astragaloside IV, astragaloside II, baohuoside-I, and astragaloside I. The total run time was 20 min. It was found that the calibration curves for all analytes showed good linearity (R-2 > 0.99) within the test ranges. The relative standard deviations for intra-and inter-day precisions were below 3.9 and 11.7%, respectively. The accuracy was evaluated by the recovery test within the range of 89.20-110.71% with the relative standard deviation < 4.8%. The sample was stable for at least 48 h at 4 degrees C. The results showed that the new approach was effective for the quality control of Bu-Shen-Yi-Qi-Fang. |
| WOS关键词 | CHEMICAL-CONSTITUENTS ; RADIX-ASTRAGALI ; IN-VITRO ; ICARIIN ; IDENTIFICATION ; GLYCOSIDES ; FORMULA ; MICE ; ISOFLAVONOIDS ; BAOHUOSIDE-1 |
| 资助项目 | National Natural Science Foundation of China[81173390] ; National 973 program of China[2009CB523000] ; Shanghai Health and Family Planning Commission[ZYSNXD-CC-HPGC-JD-015] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000345829900013 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276816]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Dong, Jingcheng |
| 作者单位 | 1.Xinjiang Uygur Med Coll, Hotan, Xinjiang, Peoples R China 2.Fudan Univ, Huashan Hosp, Dept Integrat Med, Shanghai, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Nurahmat, Mammat,Chen, Meixia,Luo, Qingli,et al. Rapid characterization and determination of multiple components in Bu-Shen-Yi-Qi-Fang by high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2014,37(23):3509-3517. |
| APA | Nurahmat, Mammat,Chen, Meixia,Luo, Qingli,Ling, Yun,Dong, Jingcheng,&Huang, Chenggang.(2014).Rapid characterization and determination of multiple components in Bu-Shen-Yi-Qi-Fang by high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry.JOURNAL OF SEPARATION SCIENCE,37(23),3509-3517. |
| MLA | Nurahmat, Mammat,et al."Rapid characterization and determination of multiple components in Bu-Shen-Yi-Qi-Fang by high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry".JOURNAL OF SEPARATION SCIENCE 37.23(2014):3509-3517. |
入库方式： OAI收割
来源：上海药物研究所
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