Identification of multiple constituents from seed of Vaccaria segetalis with an adsorbent-separation strategy based on liquid chromatography coupled to quadrupole time-of-flight mass spectrometry
文献类型：期刊论文
| 作者 | Qi, Peng; Zhang, Feng; Xue, Rui; Li, Zhixiong ; Chen, Mingcang; Sun, Zhaolin; Zhu, Kaicheng; Huang, Chenggang
|
| 刊名 | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
 |
| 出版日期 | 2014-06-15 |
| 卷号 | 28期号:11页码:1243-1257 |
| ISSN号 | 0951-4198 |
| DOI | 10.1002/rcm.6893 |
| 文献子类 | Article |
| 英文摘要 | RATIONALE Seeds of Vaccaria segetalis (Wang-Bu-Liu-Xing in Chinese) are mainly used in traditional Chinese medicine for the treatment of amenorrhea, breast infections, and edema. The study was designed to identify the components and metabolites of Wang-Bu-Liu-Xing. METHODS A novel methodology combining an adsorbent-separation strategy with analysis by liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (LC/QTOF-MS/MS) was established to identify the components of Wang-Bu-Liu-Xing. The adsorbent-separation technique was applied on macroporous resin (adsorbents). Different concentrations of ethanol (30%, 60%, and 95%), which covered high-to-low polarity ranges, were chosen as the elution solvent, respectively. The QTOF mass spectrometer was operated in negative ion mode with an electrospray ionization source. RESULTS A total of 52 components were successfully identified in the Wang-Bu-Liu-Xing decoction based on the fragmentation pathways and QTOF high-accuracy mass spectral analysis. To the best of our knowledge, several new saponins were reported for the first time. A total of 20 compounds, which included 10 prototypes and 10 metabolites, were also identified in rat plasma and urine after oral administration of Wang-Bu-Liu-Xing decoction. CONCLUSIONS An integrated adsorbent-separation strategy is powerful and reliable for global detection and identification of complex components in herbal prescriptions. The components identified in rat biofluids may also provide helpful chemical information for further pharmacology and active mechanism study on this herb. Copyright (c) 2014 John Wiley & Sons, Ltd. |
| WOS关键词 | RAT PLASMA ; TRITERPENOID SAPONINS ; MACROPOROUS RESINS ; CYCLIC-PEPTIDES ; EXTRACTION ; PURIFICATION ; METABOLITES ; COMPONENTS ; MEMBRANE ; PROTEIN |
| 资助项目 | National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09301-001] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09308-005] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
| 语种 | 英语 |
| 出版者 | WILEY-BLACKWELL |
| WOS记录号 | WOS:000337510800009 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277022]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Huang, Chenggang |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Peng,Zhang, Feng,Xue, Rui,et al. Identification of multiple constituents from seed of Vaccaria segetalis with an adsorbent-separation strategy based on liquid chromatography coupled to quadrupole time-of-flight mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2014,28(11):1243-1257. |
| APA | Qi, Peng.,Zhang, Feng.,Xue, Rui.,Li, Zhixiong.,Chen, Mingcang.,...&Huang, Chenggang.(2014).Identification of multiple constituents from seed of Vaccaria segetalis with an adsorbent-separation strategy based on liquid chromatography coupled to quadrupole time-of-flight mass spectrometry.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,28(11),1243-1257. |
| MLA | Qi, Peng,et al."Identification of multiple constituents from seed of Vaccaria segetalis with an adsorbent-separation strategy based on liquid chromatography coupled to quadrupole time-of-flight mass spectrometry".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 28.11(2014):1243-1257. |
入库方式： OAI收割
来源：上海药物研究所
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