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In Vivo Metabolism Study of Timosaponin BIII in Rat Using HPLC-QTOF-MS/MS

文献类型：期刊论文

作者Li, Dandan1,2; Xue, Rui1; Li, Zhixiong1; Chen, Mingcang1; Jiang, Weixin2; Huang, Chenggang1
刊名CHROMATOGRAPHIA
出版日期2014-06
卷号77期号:11-12页码:853-858
关键词HPLC-QTOF-MS/MS Timosaponin BIII Rat Metabolites
ISSN号0009-5893
DOI10.1007/s10337-014-2681-1
文献子类Article
英文摘要Timosaponin BIII, as one of the steroid saponins isolated from Anemarrhena asphodeloides Bge., was proved to have many pharmacological activities in recent years and became a natural active compound with good development prospect. In the present study, a simple and rapid method using high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry was developed for the determination of the structures of timosaponin BIII and its metabolites in rats after administrating intragastrically at 300 mg kg(-1). By comparing their changes in molecular masses (Delta M), retention times and spectral patterns with those of the parent compound, nine metabolites were detected and identified in urine, and eight in plasma as well as four in brain. It is also indicated that the deglycosylation and oxidation reactions were the main metabolic pathways in the biotransformation of timosaponin BIII in vivo and the structures of the nine metabolites were identified and proposed to be timosaponin BII(M1), the hydroxylated metabolite of TBII(M2), the hydroxylated metabolites of TBIII(M3 and M4), deglycosylation and monooxygenation product of TBIII(M5), the deglycosylation product of TBII(M6), timosaponin AIII(M8), the isomers of timosaponin AIII(M7 and M9).
WOS关键词ANEMARRHENA-ASPHODELOIDES ; STEROIDAL SAPONINS ; MASS-SPECTROMETRY ; PROFILE ; RHIZOMA ; URINE
资助项目National Natural Science Foundation of China[81030065] ; National Natural Science Foundation of China[81274055]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000337158600012
出版者SPRINGER HEIDELBERG
源URL[http://119.78.100.183/handle/2S10ELR8/277049]  
专题上海中药现代化研究中心
通讯作者Huang, Chenggang
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Media, Shanghai 201203, Peoples R China;
2.Harbin Univ Commerce, Harbin 150076, Peoples R China
推荐引用方式
GB/T 7714		 Li, Dandan,Xue, Rui,Li, Zhixiong,et al. In Vivo Metabolism Study of Timosaponin BIII in Rat Using HPLC-QTOF-MS/MS[J]. CHROMATOGRAPHIA,2014,77(11-12):853-858.
APA Li, Dandan,Xue, Rui,Li, Zhixiong,Chen, Mingcang,Jiang, Weixin,&Huang, Chenggang.(2014).In Vivo Metabolism Study of Timosaponin BIII in Rat Using HPLC-QTOF-MS/MS.CHROMATOGRAPHIA,77(11-12),853-858.
MLA Li, Dandan,et al."In Vivo Metabolism Study of Timosaponin BIII in Rat Using HPLC-QTOF-MS/MS".CHROMATOGRAPHIA 77.11-12(2014):853-858.

入库方式： OAI收割

来源：上海药物研究所

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