High-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry for Metabolism Study of Timosaponin AIII
文献类型：期刊论文
| 作者 | Jia, Yao1,2; Wu, Bin2; Fan, Mingsong2; Wang, Jinhui1; Huang, Jian1; Huang, Chenggang2
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| 刊名 | JOURNAL OF CHROMATOGRAPHIC SCIENCE
 |
| 出版日期 | 2014-05 |
| 卷号 | 52期号:5页码:418-422 |
| ISSN号 | 0021-9665 |
| DOI | 10.1093/chromsci/bmt052 |
| 文献子类 | Article |
| 英文摘要 | A sensitive method based on high-performance liquid chromatography-electrospray ionization tandem mass spectrometry was developed for the determination of timosaponin AIII (TA3) and its in vivo and in vitro metabolites. The rat plasma, urine, feces and tissue samples were collected after oral administration of TA3 at a single dose of 300 mg/kg. TA3 was incubated into artificial gastric juice and artificial intestinal juice. The in vivo and in vitro samples were purified by using liquid-liquid extraction. The structures of metabolites were elucidated by comparing their molecular weights, retention times and tandem mass spectrometric spectra with those of the parent drug. As a result, four metabolites (deglycosylated TA3, two hydroxylated TA3 and timosaponin BII) and the parent drug were found in in vivo and in vitro samples. In addition to the parent drug, one, one and two metabolites were identified in heart, urine and feces, respectively. Only the parent drug was detected in plasma, liver and kidney. One hydroxylation metabolite and TA3 were identified from incubation samples with AGJ, whereas two hydroxylation metabolites and TA3 were detected from the incubation with AIJ. This is the first systematic metabolism study of TA3. The biotransformation pathways of TA3 primarily included deglycosylation, hydroxylation and glycosylation. |
| WOS关键词 | RAT URINE ; ANEMARRHENA-ASPHODELOIDES ; STEROIDAL SAPONINS ; A-III ; IDENTIFICATION ; RHIZOMA ; MS/MS |
| 资助项目 | National Science and Technology Major Project Key New Drug Creation and Manufacturing Program of China[2012ZX09301001-001] ; National Natural Science Foundation of China[81030065] ; National Natural Science Foundation of China[81274055] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000342734600008 |
| 出版者 | OXFORD UNIV PRESS INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277084]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Huang, Jian |
| 作者单位 | 1.Shenyang Pharmaceut Univ, Coll Chinese Tradit Mat Med, Shenyang, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Yao,Wu, Bin,Fan, Mingsong,et al. High-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry for Metabolism Study of Timosaponin AIII[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2014,52(5):418-422. |
| APA | Jia, Yao,Wu, Bin,Fan, Mingsong,Wang, Jinhui,Huang, Jian,&Huang, Chenggang.(2014).High-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry for Metabolism Study of Timosaponin AIII.JOURNAL OF CHROMATOGRAPHIC SCIENCE,52(5),418-422. |
| MLA | Jia, Yao,et al."High-Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry for Metabolism Study of Timosaponin AIII".JOURNAL OF CHROMATOGRAPHIC SCIENCE 52.5(2014):418-422. |
入库方式： OAI收割
来源：上海药物研究所
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