HPLC-Q-TOF-MS/MS for analysis of major chemical constituents of Yinchen-Zhizi herb pair extract
文献类型：期刊论文
| 作者 | Fu, Zhiwen1,2; Ling, Yun1; Li, Zhixiong1 ; Chen, Mingcang1; Sun, Zhaolin1; Huang, Chenggang1
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| 刊名 | BIOMEDICAL CHROMATOGRAPHY
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| 出版日期 | 2014-04 |
| 卷号 | 28期号:4页码:475-485 |
| 关键词 | major chemical constituents identification Yinchen-Zhizi herb pair HPLC-Q-TOF-MS MS |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.3057 |
| 文献子类 | Article |
| 英文摘要 | The Yinchen-Zhizi herb pair (YZHP) consists of Herba Artemisiae Scopariae (Yinchen in Chinese) and Fructus Gardeniae (Zhizi in Chinese), and is mainly used to treat icteric hepatitis, itching skin and eczema. However, the bioactive constituents responsible for the pharmacological effects of YZHP are still unclear to date. In this work, a rapid and sensitive method was established to comprehensively study the constituents in YZHP extract by HPLC-Q-TOF MS/MS. The analysis was performed on an HPLC system equipped with an Agilent poroshell 120 EC-C-18 column (100x2.1mm, 2.7mm) working in a gradient elution program coupled to quadrupole-time-of-flight mass spectrometry operating in the negative ion mode. As a result, a total of 46 compounds including 17 from Herba Artemisiae Scopariae and 36 from Fructus Gardeniae were detected and tentatively identified in YZHP extract by comparing the retention time and mass spectrometry and retrieving the reference literature. More importantly, a series of constituents, such as many iridoid glycosides, were reported for the first time in this formula. The HPLC-Q-TOF MS/MS method was developed and utilized successfully to identify the major constituents in YZHP extract and would be helpful for further metabolism and pharmacology research on YZHP. Copyright (c) 2013 John Wiley & Sons, Ltd. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; ZI-CHI DECOCTION ; CHEN-HAO-TANG ; RAT PLASMA ; GARDENIA-JASMINOIDES ; IRIDOID GLYCOSIDES ; CHLOROGENIC ACIDS ; IDENTIFICATION ; MEDICINE ; FRUIT |
| 资助项目 | National Natural Science Foundation of China[90209042] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2012ZX09301001-001] ; Shanghai Committee of Science and Technology[13401900303] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000334331700005 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277134]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Huang, Chenggang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Shanghai Univ Tradit Chinese Med, Dept Clin Pharmacol, Shuguang Hosp, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Zhiwen,Ling, Yun,Li, Zhixiong,et al. HPLC-Q-TOF-MS/MS for analysis of major chemical constituents of Yinchen-Zhizi herb pair extract[J]. BIOMEDICAL CHROMATOGRAPHY,2014,28(4):475-485. |
| APA | Fu, Zhiwen,Ling, Yun,Li, Zhixiong,Chen, Mingcang,Sun, Zhaolin,&Huang, Chenggang.(2014).HPLC-Q-TOF-MS/MS for analysis of major chemical constituents of Yinchen-Zhizi herb pair extract.BIOMEDICAL CHROMATOGRAPHY,28(4),475-485. |
| MLA | Fu, Zhiwen,et al."HPLC-Q-TOF-MS/MS for analysis of major chemical constituents of Yinchen-Zhizi herb pair extract".BIOMEDICAL CHROMATOGRAPHY 28.4(2014):475-485. |
入库方式： OAI收割
来源：上海药物研究所
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