A single, multi-faceted, enhanced strategy to quantify the chromatographically diverse constituents in the roots of Euphorbia kansui
文献类型：期刊论文
| 作者 | Hou, Jin-Jun1,2 ; Wu, Wan-Ying1; Liang, Jian3; Yang, Zhou1; Long, Hua-Li1; Cai, Lu-Ying1; Fang, Lin2; Wang, Dan-Dan1; Yao, Shuai1; Liu, Xuan1
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| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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| 出版日期 | 2014-01-25 |
| 卷号 | 88页码:321-330 |
| 关键词 | Single standard to determine multi-components (SSDMC) Euphorbia kansui Exogenous reference standard Ingenane diterpenoids Jatrophane diterpenoids |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2013.08.049 |
| 文献子类 | Article |
| 英文摘要 | Kansui radix is a famous poisonous traditional Chinese medicine. However, due to its different types of constituents with broad polarity, a variety of UV absorptions and lack of the reference standards, it was difficult to simultaneously determine the main component in kanui radix. A single, multi-faceted, enhanced strategy, exogenous reference standard - single standard to determine multi-components method (ERS-SSDMC), was proposed. Thirteen major components of kansui radix, including three jatrophane diterpenoids, eight ingenane diterpenoids and two triterpenes, among which there were three pairs of isomers, were simultaneously assayed. A C8 column, packed with 2.7 2.7 mu m core-shell particles, was optimized to separate these constituents in 25 min on HPLC instrument detected at a program wavelength. Ethyl benzoate employed as single exogenous reference standard. The method was fully validated with respect to linearity (r(2) > 0.9995), LOQs (0.1-0.41 mu g/mL), precision, accuracy (92-114%, RSD < 4.4%) and stability. The robustness of the method was performed by Plackett-Burmantest tests which eight primary chromatographic parameters were investigated. It was found that the two factors, wavelength and flow rate, should be strictly controlled. A total of 75 batches of kansui radix and its three different processing products were successfully analyzed and discriminated by applying the proposed method. This work demonstrates an effective strategy for the SSDMC method making the simultaneous assay of complex multi-component TCM system achievable. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS关键词 | STANDARD |
| 资助项目 | National Natural Science Foundation of China[81073026] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000329532800043 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277221]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Wan-Ying |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China; 3.Shenyang Pharmaceut Univ, Shenyang 110016, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Jin-Jun,Wu, Wan-Ying,Liang, Jian,et al. A single, multi-faceted, enhanced strategy to quantify the chromatographically diverse constituents in the roots of Euphorbia kansui[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2014,88:321-330. |
| APA | Hou, Jin-Jun.,Wu, Wan-Ying.,Liang, Jian.,Yang, Zhou.,Long, Hua-Li.,...&Guo, De-An.(2014).A single, multi-faceted, enhanced strategy to quantify the chromatographically diverse constituents in the roots of Euphorbia kansui.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,88,321-330. |
| MLA | Hou, Jin-Jun,et al."A single, multi-faceted, enhanced strategy to quantify the chromatographically diverse constituents in the roots of Euphorbia kansui".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 88(2014):321-330. |
入库方式： OAI收割
来源：上海药物研究所
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