An integrated exact mass spectrometric strategy for comprehensive and rapid characterization of phenolic compounds in licorice
文献类型：期刊论文
| 作者 | Xu, Tongtong1; Yang, Min1; Li, Yifeng1; Chen, Xin1; Wang, Qiurong1; Deng, Wangping1; Pang, Xiayan1; Yu, Kate2; Jiang, Baohong1 ; Guan, Shuhong1
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| 刊名 | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
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| 出版日期 | 2013-11-15 |
| 卷号 | 27期号:21页码:2297-2309 |
| ISSN号 | 0951-4198 |
| DOI | 10.1002/rcm.6696 |
| 文献子类 | Article |
| 英文摘要 | RATIONALELicorice (Gancao) is derived from the dried roots and rhizomes of Glycyrrhiza species (Leguminosae) and appears as a component herb in about 60% of traditional Chinese medicine (TCM) prescriptions. Modern pharmacological studies have shown that flavonoids are one class of the major components responsible for the bioactivities of licorice. Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/QTOF MS) has proven to be a powerful tool for rapid profiling and identification of natural products in complex herbal medicines. METHODSA UPLC/QTOF MS method was established for the first time for profiling and structural characterization of the phenolic compounds (most of them flavonoids) in licorice. The combined use of data-independent acquisition (MSE) and data-dependent acquisition (DDA) was illustrated. RESULTSFifteen flavonoid reference compounds were used to explore the fragmentation pathways. Compound identification was based upon the exact mass, general fragmentation behaviors, retention times, UV absorption, and the related botanical biogenesis. As a result, a total of 51 compounds were characterized, three of which were reported for the first time. CONCLUSIONSThe LC/MS analysis for each injection took less than 9 min. The developed method is fast, accurate and reliable due to its high resolution and high efficiency characteristics as a result of combining both UPLC separation and QTOF exact mass measurement. Copyright (c) 2013 John Wiley & Sons, Ltd. |
| WOS关键词 | HAIRY ROOT CULTURES ; GLYCYRRHIZA-GLABRA ; FLAVONOID CONSTITUENTS ; TRITERPENE SAPONINS ; IN-VITRO ; IDENTIFICATION ; HPLC ; ELUCIDATION ; GLYCOSIDES ; EXTRACT |
| 资助项目 | National Natural Science Foundation of China[81001630] ; Natural Science Foundation of Shanghai[10ZR1436400] ; Major Projects of Knowledge Innovation Program of the Chinese Academy of Sciences[KSCX2-YW-R-166] ; Twelfth Five-Year National Science & Technology Support Program[2012BAI29B06] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000325254300002 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277376]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Guo, De-an |
| 作者单位 | 1.Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China; 2.Waters Corp, Milford, MA 01757 USA |
| 推荐引用方式 GB/T 7714 | Xu, Tongtong,Yang, Min,Li, Yifeng,et al. An integrated exact mass spectrometric strategy for comprehensive and rapid characterization of phenolic compounds in licorice[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2013,27(21):2297-2309. |
| APA | Xu, Tongtong.,Yang, Min.,Li, Yifeng.,Chen, Xin.,Wang, Qiurong.,...&Guo, De-an.(2013).An integrated exact mass spectrometric strategy for comprehensive and rapid characterization of phenolic compounds in licorice.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,27(21),2297-2309. |
| MLA | Xu, Tongtong,et al."An integrated exact mass spectrometric strategy for comprehensive and rapid characterization of phenolic compounds in licorice".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 27.21(2013):2297-2309. |
入库方式： OAI收割
来源：上海药物研究所
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