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Elution-extrusion Counter-current Chromatography Separation of Two New Benzyl Ester Glucosides and Three Other High-polarity Compounds from the Tubers of Pleione bulbocodioides

文献类型：期刊论文

作者Wang, Yang1,2; Guan, Shu-hong2; Feng, Rui-hong2; Zhang, Jing-xian1,2; Li, Qing1; Chen, Xiao-hui1; Bi, Kai-shun1; Guo, De-an1,2
刊名PHYTOCHEMICAL ANALYSIS
出版日期2013-11
卷号24期号:6页码:671-676
关键词Counter-current chromatography high-polarity benzyl ester glucosides gastrodin Pleione bulbocodioides
ISSN号0958-0344
DOI10.1002/pca.2453
文献子类Article
英文摘要IntroductionThe tubers of Pleione bulbocodioides (Franch.) Rolfe, with gastrodin and benzyl ester glucosides as main components, have been used in traditional Chinese medicine for the treatment of various cancers and bacterial infections. Up to now, their official quality control method is still inadequate, and the difficulty of obtaining these high-polarity compounds is one of the major reasons. ObjectiveTo develop a rapid and efficient method for preparative separation of the high-polarity compounds gastrodin and benzyl ester glucosides. MethodsAn optimised solvent system composed of n-butanol:ethanol:water (20:1:20, v/v/v) was applied for the elution-extrusion counter-current chromatography (EECCC) separation. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5mL/min, a rotation speed of 850rpm and a temperature of 35 degrees C. ResultsFive high-polarity glucosides, including two new compounds, (E)-4--D-glucopyranosyloxycinnamic acid 9-(4--D-glucopyranosyloxybenzyl) ester (4mg) and (Z)-2-(2-methylpropyl)butenedioic acid bis(4--D-glucopyranosyloxybenzyl) ester (9mg), and three main components, gastrodin (87mg), dactylorhin A (60mg) and militarine (15mg), with HPLC purities of 95.4%, 96.4%, 91.1%, 97.2% and 95.5% respectively, were yielded from 400mg of the prepared sample. ConclusionElution-extrusion counter-current chromatography could be used as a useful tool for the separation of high-polarity compounds such as gastrodin and benzyl ester glucosides and the enrichment of the minor ones. Copyright (c) 2013 John Wiley & Sons, Ltd. The tubers of Pleione bulbocodioides (Franch.) Rolfe, as a traditional Chinese medicine, have been used for the treatment of various cancers and bacterial infections. Elution-extrusion counter-current chromatography (EECCC), a support-free counter-current chromatography technique, showed its great advantage in separation of high-polarity compounds. In this research, we successfully used EECCC as a useful tool for the separation of gastrodin and benzyl ester glucosides, including two new ones from this herbal medicine.
WOS关键词PREPARATIVE SEPARATION ; GASTRODIN ; CONSTITUENTS ; GLYCOSIDES ; NEURONS
资助项目Twelfth Five-Year National Science and Technology Support Program[2012BAI29B06] ; Chinese Academy of Sciences[KSCX2-YW-R-166] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2011ZX09307-002-03]
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry
语种英语
WOS记录号WOS:000326032100021
出版者WILEY-BLACKWELL
源URL[http://119.78.100.183/handle/2S10ELR8/277407]  
专题上海中药现代化研究中心
通讯作者Guo, De-an
作者单位1.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China;
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Yang,Guan, Shu-hong,Feng, Rui-hong,et al. Elution-extrusion Counter-current Chromatography Separation of Two New Benzyl Ester Glucosides and Three Other High-polarity Compounds from the Tubers of Pleione bulbocodioides[J]. PHYTOCHEMICAL ANALYSIS,2013,24(6):671-676.
APA Wang, Yang.,Guan, Shu-hong.,Feng, Rui-hong.,Zhang, Jing-xian.,Li, Qing.,...&Guo, De-an.(2013).Elution-extrusion Counter-current Chromatography Separation of Two New Benzyl Ester Glucosides and Three Other High-polarity Compounds from the Tubers of Pleione bulbocodioides.PHYTOCHEMICAL ANALYSIS,24(6),671-676.
MLA Wang, Yang,et al."Elution-extrusion Counter-current Chromatography Separation of Two New Benzyl Ester Glucosides and Three Other High-polarity Compounds from the Tubers of Pleione bulbocodioides".PHYTOCHEMICAL ANALYSIS 24.6(2013):671-676.

入库方式： OAI收割

来源：上海药物研究所

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