Analysis of Multiple Constituents in Cong-Ming-Tang, a Chinese Herbal Formula for the Treatment of Amnesia, by High-performance Liquid Chromatography with Quadrupole Time-of-flight Mass Spectrometry
文献类型：期刊论文
| 作者 | Ling, Yun; Li, Zhixiong ; Chen, Mingcang; Sun, Zhaolin; Fan, Mingsong; Huang, Chenggang
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| 刊名 | PHYTOCHEMICAL ANALYSIS
 |
| 出版日期 | 2013-11 |
| 卷号 | 24期号:6页码:677-688 |
| 关键词 | HPLC-QTOF/MS/MS chemical constituents Cong-Ming-Tang traditional Chinese medicine formula |
| ISSN号 | 0958-0344 |
| DOI | 10.1002/pca.2454 |
| 文献子类 | Article |
| 英文摘要 | IntroductionCong-Ming-Tang (CMT), named smart-soup in English, is a well-known traditional Chinese medicine formula for the treatment of amnesia in China. However, the isolation, purification and identification procedures of the major bioactive constituents in CMT are difficult and time consuming. ObjectiveTo establish a rapid and sensitive high-performance liquid chromatography/electrospray ionisation with quadrupole time-of-flight tandem mass spectrometry (HPLC-QTOF/MS/MS) method that could be applied to rapidly separate and identify the major bioactive constituents in CMT. MethodsMethanolic extract of CMT was used for HPLC-QTOF/MS/MS analysis. Separation was performed on an Agilent Poroshell 120 EC- C18 column (2.7 x100 mm .i.d., 2.7 mu m) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Both positive and negative ion modes were employed. ResultsThis analytical tool allowed the identification of 55 compounds from CMT formulae by comparing their retention times and MS spectra with those of authentic compounds or literature data in both positive and negative ion modes, including 4 xanthone C-glycosides, 4 sucrose esters, 11 oligosaccharide multi-esters,15 triterpene saponins, 15 triterpene acids, 2 lignans and 4 phenylpropanoids. Onjisaponin MF was tentatively elucidated as a new triterpene saponin based on the summarised fragmentation rules. ConclusionHPLC-QTOF/MS/MS provides a new powerful approach to identify the major chemical constituents in CMT rapidly and accurately. This study proposed a series of potential bioactive components without preparative isolation from the crude extract of CMT and indicated that the HPLC-QTOF/MS/MS method also can be a promising tool for the analysis of other traditional Chinese medicines. Copyright (c) 2013 John Wiley & Sons, Ltd. This analytical tool allowed the identification of 55 compounds from CMT formulae by comparing their retentionylpropanoids. Onjisaponin MF was tentatively elucidated as a new triterpene saponin based on the summarised fragmentation rules. n times and MS spectra with those of authentic compounds or literature data in both positive and negative ion modes, including 4 xanthone C-glycosides, 4 sucrose esters, 11 oligosaccharide multi-esters,15 triterpene saponins, 15 triterpene acids, 2 lignans and 4 phe. |
| WOS关键词 | POLYGALA-TENUIFOLIA WILLD ; PORIA-COCOS ; TRITERPENE SAPONINS ; SURFACE-LAYER ; GLYCOSIDES ; ROOTS ; ESTERS |
| 资助项目 | National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2012ZX09301001-001] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09301-001] ; Key Program of the Science and Technology Commission of Shanghai Municipality, China[09JC1416400] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000326032100022 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277408]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Huang, Chenggang |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ling, Yun,Li, Zhixiong,Chen, Mingcang,et al. Analysis of Multiple Constituents in Cong-Ming-Tang, a Chinese Herbal Formula for the Treatment of Amnesia, by High-performance Liquid Chromatography with Quadrupole Time-of-flight Mass Spectrometry[J]. PHYTOCHEMICAL ANALYSIS,2013,24(6):677-688. |
| APA | Ling, Yun,Li, Zhixiong,Chen, Mingcang,Sun, Zhaolin,Fan, Mingsong,&Huang, Chenggang.(2013).Analysis of Multiple Constituents in Cong-Ming-Tang, a Chinese Herbal Formula for the Treatment of Amnesia, by High-performance Liquid Chromatography with Quadrupole Time-of-flight Mass Spectrometry.PHYTOCHEMICAL ANALYSIS,24(6),677-688. |
| MLA | Ling, Yun,et al."Analysis of Multiple Constituents in Cong-Ming-Tang, a Chinese Herbal Formula for the Treatment of Amnesia, by High-performance Liquid Chromatography with Quadrupole Time-of-flight Mass Spectrometry".PHYTOCHEMICAL ANALYSIS 24.6(2013):677-688. |
入库方式： OAI收割
来源：上海药物研究所
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