Analysis and detection of the chemical constituents of Radix Polygalae and their metabolites in rats after oral administration by ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry
文献类型：期刊论文
| 作者 | Ling, Yun; Li, Zhixiong ; Chen, Mingcang; Sun, Zhaolin; Fan, Mingsong; Huang, Chenggang
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| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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| 出版日期 | 2013-11 |
| 卷号 | 85页码:1-13 |
| 关键词 | Radix Polygalae UHPLC/ESI-Q-TOF-MS Chemical constituents Metabolites |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2013.06.011 |
| 文献子类 | Article |
| 英文摘要 | Radix Polygalae (RP), the dried root of Polygala tenuifolia Willd., is a well-known traditional Chinese medicine to mediate sedative, antipsychotic, cognitive improving, neuroprotective, and anti-inflammatory therapeutic effects on the central nervous system. In this work, ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC/ESI-Q-TOF-MS/MS) was established for the separation and characterization of the chemical constituents in Radix Polygalae and their metabolites in rat plasma and urine after oral administration. Samples were separated on an Agilent Zorbax Eclipse Plus-C18 column (100 mm x 2.1 mm, 1.8 mu m) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Overall, 50 compounds were characterized from the RP, 9 of which are to our knowledge reported for the first time. In vivo, 10 components and 2 metabolites were observed in rat plasma, and 27 components and 7 metabolites were detected in rat urine. The results from this work improve our understanding on the chemical constituents of RP and their metabolic profiling. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS关键词 | TENUIFOLIA WILLD ; TRITERPENE SAPONINS ; SUCROSE ESTERS ; ROOT ; GLYCOSIDES ; XANTHONES ; MEMORY ; XXXII |
| 资助项目 | National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2012ZX09301001-001] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09301-001] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09501-030] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09103-373] ; Science and Technology Commission of Shanghai Municipality, China[09JC1416400] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000324351200001 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277412]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Huang, Chenggang |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ling, Yun,Li, Zhixiong,Chen, Mingcang,et al. Analysis and detection of the chemical constituents of Radix Polygalae and their metabolites in rats after oral administration by ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2013,85:1-13. |
| APA | Ling, Yun,Li, Zhixiong,Chen, Mingcang,Sun, Zhaolin,Fan, Mingsong,&Huang, Chenggang.(2013).Analysis and detection of the chemical constituents of Radix Polygalae and their metabolites in rats after oral administration by ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,85,1-13. |
| MLA | Ling, Yun,et al."Analysis and detection of the chemical constituents of Radix Polygalae and their metabolites in rats after oral administration by ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 85(2013):1-13. |
入库方式： OAI收割
来源：上海药物研究所
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