Metabolism and tissue distribution study of Vaccaria seeds (Wang-Bu-Liu-Xing) in benign prostatic hyperplasia model rat: Toward an in-depth study for its bioactive components
文献类型：期刊论文
| 作者 | Qi, Peng; Li, Zhixiong ; Chen, Mingcang; Sun, Zhaolin; Huang, Chenggang
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| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2013-11 |
| 卷号 | 85页码:218-230 |
| ISSN号 | 0731-7085 |
| 关键词 | Wang-Bu-Liu-Xing LC-QTOF-MS/MS Metabolism Tissue distribution BPH-model rat |
| DOI | 10.1016/j.jpba.2013.07.037 |
| 文献子类 | Article |
| 英文摘要 | Vaccaria seeds (Wang-Bu-Liu-Xing), a well-known traditional Chinese medicine (TCM), has been used as an emperor herb of many ancient formulas to treat benign prostatic hyperplasia (BPH) in clinic. However, its metabolism and tissue distribution, especially in the target tissue, had not been investigated so far. Based on the hypothesis that the components which exert effect against BPH of Vaccaria seeds would be measureable in target tissue (prostate), in vivo metabolism and tissue distribution of Vaccaria seeds in rats were profiled using a specific and sensitive high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (LC-QTOF-MS/MS). As a result, 19 major constituents in the Vaccaria seeds decoction and 19 constituents in rat plasma, feces and tissues after oral administration of Vaccaria seeds decoction were identified. Accurate mass measurement for molecular ions and characteristic fragment ions could represent reliable identification criteria for these compounds. Two prototypes were detected in prostate. An in vitro metabolism analysis of them was studied after incubation with rat intestinal flora and rat liver microsome (RLM) in this paper, which is helpful for further investigation of the potential effect of these two components. The result of this study provided meaningful information for further pharmacology research on Vaccaria seeds. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS关键词 | TRITERPENOID SAPONINS ; CYCLIC-PEPTIDES ; RAPID CHARACTERIZATION ; SEGETALIS ; MICE ; CONSTITUENTS ; FLAVONOIDS ; SEPARATION ; GROWTH |
| 资助项目 | National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09301-001] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09308-005] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000324351200030 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277413]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Huang, Chenggang |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Peng,Li, Zhixiong,Chen, Mingcang,et al. Metabolism and tissue distribution study of Vaccaria seeds (Wang-Bu-Liu-Xing) in benign prostatic hyperplasia model rat: Toward an in-depth study for its bioactive components[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2013,85:218-230. |
| APA | Qi, Peng,Li, Zhixiong,Chen, Mingcang,Sun, Zhaolin,&Huang, Chenggang.(2013).Metabolism and tissue distribution study of Vaccaria seeds (Wang-Bu-Liu-Xing) in benign prostatic hyperplasia model rat: Toward an in-depth study for its bioactive components.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,85,218-230. |
| MLA | Qi, Peng,et al."Metabolism and tissue distribution study of Vaccaria seeds (Wang-Bu-Liu-Xing) in benign prostatic hyperplasia model rat: Toward an in-depth study for its bioactive components".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 85(2013):218-230. |
入库方式： OAI收割
来源：上海药物研究所
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