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Pharmacokinetics of ganoderic acid D and its main metabolite by liquid chromatography-tandem mass spectrometry

文献类型：期刊论文

作者Cheng, Chun-Ru1,2; Yang, Min1; Guan, Shu-Hong1; Wu, Xiao-Hui3; Pang, Xiao-Yan1; Wang, Yang1; Yang, Yi2; Ding, Jie2; Guo, De-An1
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期2013-07-01
卷号930页码:1-6
关键词Ganoderic acid D Ganoderic acid B LC/MS/MS Nanoparticle Pharmacokinetics Triterpenoid
ISSN号1570-0232
DOI10.1016/j.jchromb.2013.04.015
文献子类Article
英文摘要The present study aims to investigate the pharmacokinetics of ganoderic acid D (GD), a representative active triterpenoid from Ganoderrna lucidum. A sensitive and selective liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of the concentrations of GD and its main metabolite (ganoderic acid B) in rat plasma. Following protein precipitation, the analytes were separated on a reversed-phase C18 column. Acetonitrile-water-acetic acid (40:60:0.01) was used at a flow-rate of 0.2 ml/min. A triple quadrupole mass spectrometer equipped with an electro-spray ionization source was used as the detector and was operated in the negative ion mode. Multiple reaction monitoring using the characteristic transitions was performed to quantify the analytes. The method had a lower limit of quantification of 8.19 ng/ml for GD, and 8.59 ng/ml for ganoderic acid B (GB). The calibration curves were demonstrated to be linear over the concentration range of 8.19-4096 ng/ml and 8.59-2149 ng/ml, respectively. Variations within- and between-batch were less than 6.4% and 4.6%, respectively. The extraction recovery rates ranged from 98.8 to 105.2% and 100.7 to 113.6%, respectively. The validated method was successfully applied to the quantification of GD and GB concentrations in rat plasma after oral administration (or intravenous administration) of GD preparations at a dose of 15 mg/kg. The data showed that the absolute bioavailability increased from 22% to 70% after the GD suspension was changed to GD loaded solid lipid nanoparticles. In the meantime, the C-max increased from 107.2 to 1555.6 ng/ml; the t(max) changed from 2.0 h to 0.3 h. These results are very helpful in the further studies. (C) 2013 Elsevier B.V. All rights reserved.
WOS关键词LUCIDUM ; TRITERPENOIDS
资助项目National Natural Science Foundation of China[81001630] ; Natural Science Foundation of Shanghai[10ZR1436400] ; Chinese Academy of Sciences[KSCX2-YW-R-166] ; Twelfth Five-Year National Science & Technology Support Program[2012BAI29B06] ; Major Project of Zigong Municipal and Technology Bureau[11GC016] ; Sichuan University of Science Engineering[2012RC17] ; Sichuan University of Science Engineering[2012RC15]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000320744800001
出版者ELSEVIER SCIENCE BV
源URL[http://119.78.100.183/handle/2S10ELR8/277559]  
专题上海中药现代化研究中心
通讯作者Yang, Min
作者单位1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med,Natl Engn Lab TCM Standardi, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China;
2.Sichuan Univ Sci & Engn, Sch Chem & Pharmaceut Engn, Zigong 643000, Sichuan, Peoples R China;
3.Tianjin Med Univ, Coll Pharm, Tianjin Key Lab Technol Enabling Dev Clin Therape, Tianjin 300070, Peoples R China
推荐引用方式
GB/T 7714		 Cheng, Chun-Ru,Yang, Min,Guan, Shu-Hong,et al. Pharmacokinetics of ganoderic acid D and its main metabolite by liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,930:1-6.
APA Cheng, Chun-Ru.,Yang, Min.,Guan, Shu-Hong.,Wu, Xiao-Hui.,Pang, Xiao-Yan.,...&Guo, De-An.(2013).Pharmacokinetics of ganoderic acid D and its main metabolite by liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,930,1-6.
MLA Cheng, Chun-Ru,et al."Pharmacokinetics of ganoderic acid D and its main metabolite by liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 930(2013):1-6.

入库方式： OAI收割

来源：上海药物研究所

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