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Identification of the Major Constituents in Zhimu-Huangqi Herb-Pair Extract and Their Metabolites in Rats by LC-ESI-MSn

文献类型：期刊论文

作者Li, Zhixiong1,2; Song, Xinmeng2,3; Fu, Zhiwen1,2; Wu, Bin2; Ling, Yun2; Sun, Zhaolin2; Chen, Mingcang2; Xu, Desheng1; Huang, Chenggang2
刊名CHROMATOGRAPHIA
出版日期2013-07
卷号76期号:13-14页码:767-780
关键词LC-ESI-MSn Rhizoma Anemarrhenae and Radix Astragali Major constituents Metabolites
ISSN号0009-5893
DOI10.1007/s10337-013-2475-x
文献子类Article
英文摘要The Zhimu-Huangqi herb-pair is a famous Chinese herbal formula with a combination of Rhizoma Anemarrhenae (Zhimu in Chinese) and Radix Astragali (Huangqi in Chinese). This work describes a sensitive and specific LC-ES-MSn methodology for identification of the major constituents in Zhimu-Huangqi herb-pair extract and their metabolites in rats after oral administration. A total of 30 compounds have been identified or tentatively characterized from the herb-pair extract, and 13 of them were unambiguously identified by comparing the retention times and mass spectra with those of reference standards, while the other 17 compounds were tentatively identified on the basis of their MSn fragmentation behaviors and exact mass information from literature. Moreover, the metabolites in vivo were also identified. The Zhimu-Huangqi herb-pair extract was actively metabolized in rats, including four parent compounds and 8 metabolites in serum and seven parent compounds and 23 metabolites in urine. This study proposed a good example for the rapid identification of major constituents in complex systems such as herbal extract or traditional Chinese medicine formula, which facilitated the clarification of the metabolic pathway of the herbs in the body to better understand the action mechanism.
WOS关键词PERFORMANCE LIQUID-CHROMATOGRAPHY ; FLIGHT MASS-SPECTROMETRY ; ANEMARRHENA-ASPHODELOIDES RHIZOMES ; TRADITIONAL CHINESE MEDICINES ; STEROIDAL SAPONINS ; ASTRAGALUS-MEMBRANACEUS ; RADIX-ASTRAGALI ; IN-VIVO ; DECOCTION ; MONGHOLICUS
资助项目State Key Program of the National Natural Science Foundation of China[81030065] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2012ZX09301001-001] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2013ZX09102027]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000320959000005
出版者SPRINGER HEIDELBERG
源URL[http://119.78.100.183/handle/2S10ELR8/277565]  
专题上海中药现代化研究中心
通讯作者Xu, Desheng
作者单位1.Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China;
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
3.Harbin Univ Commerce, Harbin 150076, Peoples R China
推荐引用方式
GB/T 7714		 Li, Zhixiong,Song, Xinmeng,Fu, Zhiwen,et al. Identification of the Major Constituents in Zhimu-Huangqi Herb-Pair Extract and Their Metabolites in Rats by LC-ESI-MSn[J]. CHROMATOGRAPHIA,2013,76(13-14):767-780.
APA Li, Zhixiong.,Song, Xinmeng.,Fu, Zhiwen.,Wu, Bin.,Ling, Yun.,...&Huang, Chenggang.(2013).Identification of the Major Constituents in Zhimu-Huangqi Herb-Pair Extract and Their Metabolites in Rats by LC-ESI-MSn.CHROMATOGRAPHIA,76(13-14),767-780.
MLA Li, Zhixiong,et al."Identification of the Major Constituents in Zhimu-Huangqi Herb-Pair Extract and Their Metabolites in Rats by LC-ESI-MSn".CHROMATOGRAPHIA 76.13-14(2013):767-780.

入库方式： OAI收割

来源：上海药物研究所

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