Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against paclitaxel
文献类型：期刊论文
| 作者 | Chao, Zhi1; Tan, Mingming1; Paudel, Madan Kumar2; Sakamoto, Seiichi2; Ma, Liling3; Sasaki-Tabata, Kaori2; Tanaka, Hiroyuki2; Shoyama, Yukihiro4; Xuan, Lijiang5 ; Morimoto, Satoshi2
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| 刊名 | JOURNAL OF NATURAL MEDICINES
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| 出版日期 | 2013-07 |
| 卷号 | 67期号:3页码:512-518 |
| 关键词 | Paclitaxel Monoclonal antibody ELISA Taxus |
| ISSN号 | 1340-3443 |
| DOI | 10.1007/s11418-012-0708-1 |
| 文献子类 | Article |
| 英文摘要 | Paclitaxel, the major active component of the yew tree, is used as an important anti-cancer agent. To obtain the monoclonal antibody (MAb) against paclitaxel for paclitaxel determination using immunoassay, 7-xylosyltaxol was conjugated to the carrier protein bovine serum albumin (BSA) to construct the immunogen, and the ratio of hapten in XylTax-BSA conjugate was determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. After immunization of mice with this conjugate, hybridomas secreting MAbs against paclitaxel were obtained by fusing the splenocytes with the mouse myeloma cell line SP2/0. After hybridoma screening, the anti-paclitaxel MAb 3A3 was obtained, which showed a relatively high specificity to paclitaxel (cross-reactivities against other naturally occurred taxanes: 7-xylosyltaxol, 31.8 %; cephalomannine, 6.17 %; baccatin III, 10-deacetyl-baccatin III, 1-hydroxybaccatin I, 13-acetyl-9-dihydrobaccatin III and 1-acetoxyl-5-deacetyl-baccatin I, < 0.11 %). Using the MAb 3A3, we established an indirect competitive enzyme-linked immunosorbent assay (icELISA) for paclitaxel determination with a detection range of 0.098-312.5 mu g ml(-1). Determination of paclitaxel contents in various yew tree samples with this icELISA resulted in recovery rates ranging from 92 to 94.8 %, and intra- and inter-assay variations of 3.6 and 4.7 %, respectively. This icELISA provides a valuable method of paclitaxel determination for various purposes. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; TAXUS-BREVIFOLIA EXTRACTS ; MASS-SPECTROMETRY ; HUMAN PLASMA ; QUANTITATIVE-DETERMINATION ; BIOLOGICAL-FLUIDS ; CARCINOMA CELLS ; OVARIAN-CANCER ; TAXOL ; TAXANES |
| 资助项目 | Natural Science Foundation of China (NSFC)[30500652] ; Guangdong Administration of Traditional Chinese Medicine Scientific and Technological Projects[1060131] ; Ministry of Education, Culture, Sports, Science and Technology of Japan[00000000] ; Takeda Science Foundation[00000000] ; Sasagawa Scholarship[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000320112500010 |
| 出版者 | SPRINGER JAPAN KK |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277566]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Chao, Zhi |
| 作者单位 | 1.Southern Med Univ, Sch Tradit Chinese Med, Guangzhou 510515, Guangdong, Peoples R China; 2.Kyushu Univ, Grad Sch Pharmaceut, Fukuoka 8128582, Japan; 3.Guangzhou Med Univ, Sch Nursing, Guangzhou 510450, Guangdong, Peoples R China; 4.Nagasaki Int Univ, Dept Pharm, Nagasaki 8593298, Japan; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chao, Zhi,Tan, Mingming,Paudel, Madan Kumar,et al. Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against paclitaxel[J]. JOURNAL OF NATURAL MEDICINES,2013,67(3):512-518. |
| APA | Chao, Zhi.,Tan, Mingming.,Paudel, Madan Kumar.,Sakamoto, Seiichi.,Ma, Liling.,...&Morimoto, Satoshi.(2013).Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against paclitaxel.JOURNAL OF NATURAL MEDICINES,67(3),512-518. |
| MLA | Chao, Zhi,et al."Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against paclitaxel".JOURNAL OF NATURAL MEDICINES 67.3(2013):512-518. |
入库方式： OAI收割
来源：上海药物研究所
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