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Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry

文献类型：期刊论文

作者Zeng, Feng1,2; Wang, Wei3,4; Guan, Shuhong2; Cheng, Chunru2; Yang, Min2; Avula, Bharathi5; Khan, Ikhlas A.5; Guo, De-an1,2
刊名PLANTA MEDICA
出版日期2013-06
卷号79期号:9页码:797-805
关键词Tripterygium wilfordii sesquiterpenoid alkaloids diterpenoids triterpenoids HPLC-ESI-MS multiple reaction monitoring Celastraceae
ISSN号0032-0943
DOI10.1055/s-0032-1328596
文献子类Article
英文摘要A liquid chromatography-electrospray ionization-mass spectrometry method with multiple reaction monitoring was established for simultaneous quantification of 18 bioactive constituents from the stem and root of Tripterygium wilfordii Hook. f. collected from different places in China and various commercial preparations. The chromatographic separations were achieved on an Agilent Poroshell SB-C-18 column (150 x 4.6 mm, 3.5 mu m) with gradient elution using acetonitrile and 0.03% formic acid aqueous solution in 45 min. Detection was performed in the positive ionization mode by monitoring the precursor-product combination. The validation of the method included tests of linearity, sensitivity, precision, repeatability, stability, and accuracy. All calibration curves showed good linearity (r > 0.9990) within the test range. The established method showed good precision and accuracy with intra-day and interday variations of 2-5% and 1-4%, respectively, and recoveries of 95.5-104.5%.
WOS关键词SOLID-PHASE EXTRACTION ; CHINESE HERBAL REMEDY ; THUNDER GOD VINE ; NF-KAPPA-B ; HOOK-F ; ROOT EXTRACTS ; TRIPTOLIDE ; EXPRESSION ; SUPPRESSION ; ALKALOIDS
资助项目program for Changjiang Scholars and Innovation Team in University[985-2-063-112] ; Ministry of Education of China[104218] ; Global Research Network for Medicinal Plants (GRNMP)[00000000] ; King Saud University[00000000]
WOS研究方向Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种英语
WOS记录号WOS:000320451500013
出版者GEORG THIEME VERLAG KG
源URL[http://119.78.100.183/handle/2S10ELR8/277600]  
专题上海中药现代化研究中心
通讯作者Guo, De-an
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China;
2.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Biol Sci,Shanghai Inst Mat Med, Shanghai, Peoples R China;
3.Hunan Univ Chinese Med, Sch Pharm, TCM, Changsha 410208, Hunan, Peoples R China;
4.Hunan Univ Chinese Med, Sch Pharm, Ethnomed Innovat & Dev Lab, Changsha 410208, Hunan, Peoples R China;
5.Univ Mississippi, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, Sino US TCM Res Ctr, University, MS 38677 USA
推荐引用方式
GB/T 7714		 Zeng, Feng,Wang, Wei,Guan, Shuhong,et al. Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry[J]. PLANTA MEDICA,2013,79(9):797-805.
APA Zeng, Feng.,Wang, Wei.,Guan, Shuhong.,Cheng, Chunru.,Yang, Min.,...&Guo, De-an.(2013).Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry.PLANTA MEDICA,79(9),797-805.
MLA Zeng, Feng,et al."Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry".PLANTA MEDICA 79.9(2013):797-805.

入库方式： OAI收割

来源：上海药物研究所

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