In vivo metabolism study of vaccarin in rat using HPLC-LTQ-MSn
文献类型：期刊论文
| 作者 | Qi, Peng; Fan, Mingsong; Li, Zhixiong ; Chen, Mingcang; Sun, Zhaolin; Wu, Bin; Huang, Chenggang
|
| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 2013-01 |
| 卷号 | 27期号:1页码:96-101 |
| 关键词 | vaccarin HPLC-ESI-MSn LTQ XL metabolite |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.2753 |
| 文献子类 | Article |
| 英文摘要 | In order to illustrate the main biotransformation pathways of vaccarin in vivo, metabolites of vaccarin in rats were identified using a specific and sensitive high-performance liquid chromatographyelectrospray ionization linear ion trap mass spectrometry (LTQ XL (TM)) method. The rats were administered a single dose (200?mg/kg) of vaccarin by oral gavage. By comparing their changes in molecular masses (?M), retention times and spectral patterns with those of the parent drug, the parent compound and six metabolites were found in rat urine after oral administration of vaccarin. The parent compound and five metabolites were detected in rat plasma. In heart, liver and kidney samples, respectively, one, four and three metabolites were identified, in addition to the parent compound. Three metabolites, but no trace of parent drug, were found in the rat feces. This is the first systematic metabolism study of vaccarin in vivo. The biotransformation pathways of vaccarin involved methylation, hydroxylation, glycosylation and deglycosylation. Copyright (c) 2012 John Wiley & Sons, Ltd. |
| WOS关键词 | TRITERPENOID SAPONINS ; MASS-SPECTROMETRY ; IDENTIFICATION ; SEGETALIS |
| 资助项目 | National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09301-001] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09308-005] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000312306300013 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277832]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Huang, Chenggang |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Peng,Fan, Mingsong,Li, Zhixiong,et al. In vivo metabolism study of vaccarin in rat using HPLC-LTQ-MSn[J]. BIOMEDICAL CHROMATOGRAPHY,2013,27(1):96-101. |
| APA | Qi, Peng.,Fan, Mingsong.,Li, Zhixiong.,Chen, Mingcang.,Sun, Zhaolin.,...&Huang, Chenggang.(2013).In vivo metabolism study of vaccarin in rat using HPLC-LTQ-MSn.BIOMEDICAL CHROMATOGRAPHY,27(1),96-101. |
| MLA | Qi, Peng,et al."In vivo metabolism study of vaccarin in rat using HPLC-LTQ-MSn".BIOMEDICAL CHROMATOGRAPHY 27.1(2013):96-101. |
入库方式： OAI收割
来源：上海药物研究所
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