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Identification of Metabolites of Ganoderic Acid D by Ultra-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry

文献类型：期刊论文

作者Cheng, Chunru2; Yang, Min2; Yu, Kate1; Guan, Shuhong2; Tao, Sijia3,4; Millar, Alan1; Pang, Xiaoyan2; Guo, Dean2
刊名DRUG METABOLISM AND DISPOSITION
出版日期2012-12
卷号40期号:12页码:2307-2314
ISSN号0090-9556
DOI10.1124/dmd.112.047506
文献子类Article
英文摘要Ganoderic acid D (GD) is the major active triterpenoid in Ganoderma lucidum, a medicinal fungus used daily. However, the metabolic fate of GD remains unknown. To know whether GD is extensively metabolized, we first investigated the metabolism of GD in vitro and in vivo. The metabolic profiles of the bile samples obtained from rats in vivo were almost the same as those obtained in vitro. Using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry, a total of 25 metabolites were identified from the bile sample. Few metabolites were found in the urine samples. These results indicated that biliary rather than renal clearance was the major route of excretion. The major metabolites were identified by comparison with the standard reference compounds. Metabolites at low concentrations were identified by interpreting the mass spectra. Both phase I and phase II metabolites were observed. The metabolic transformation included reduction, monohydroxylation, dihydroxylation, trihydroxylation, oxidation, desaturation, sulfation, and glucuronidation. The main metabolic soft spots in the chemical structure of GD were the 3-carbonyl group, angular methyl groups, the 7-hydroxy group, and the 26-carboxylic acid moiety. Overall, this study gives us an insight into the metabolism of GD, an active oxygenated tetracyclic triterpenoid.
WOS关键词LUCIDUM ; TRITERPENOIDS ; PRODUCTS
资助项目National Natural Science Foundation of China[30701077] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program," China[2009ZX09308-005] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program," China[2009ZX09311-001] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program," China[2009ZX09502-020] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program," China[2009ZX09304-002] ; Chinese Academy of Sciences[KSCX2-YW-R-166]
WOS研究方向Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000311106300011
出版者AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS
源URL[http://119.78.100.183/handle/2S10ELR8/277866]  
专题上海中药现代化研究中心
通讯作者Guo, Dean
作者单位1.Waters Corp, Milford, MA USA;
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol,Shanghai I, Shanghai 201203, Peoples R China;
3.Emory Univ, Sch Med, Dept Pediat, Ctr AIDS Res,Lab Biochem Pharmacol, Decatur, GA 30033 USA;
4.Vet Affairs Med Ctr, Decatur, GA 30033 USA
推荐引用方式
GB/T 7714		 Cheng, Chunru,Yang, Min,Yu, Kate,et al. Identification of Metabolites of Ganoderic Acid D by Ultra-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry[J]. DRUG METABOLISM AND DISPOSITION,2012,40(12):2307-2314.
APA Cheng, Chunru.,Yang, Min.,Yu, Kate.,Guan, Shuhong.,Tao, Sijia.,...&Guo, Dean.(2012).Identification of Metabolites of Ganoderic Acid D by Ultra-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry.DRUG METABOLISM AND DISPOSITION,40(12),2307-2314.
MLA Cheng, Chunru,et al."Identification of Metabolites of Ganoderic Acid D by Ultra-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry".DRUG METABOLISM AND DISPOSITION 40.12(2012):2307-2314.

入库方式： OAI收割

来源：上海药物研究所

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