Systematic screening and characterization of the major bioactive components of Poria cocos and their metabolites in rats by LC-ESI-MSn
文献类型：期刊论文
| 作者 | Ling, Yun1; Chen, Mingcang1; Wang, Ke2; Sun, Zhaolin1; Li, Zhixiong1 ; Wu, Bin1; Huang, Chenggang1
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| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 2012-09 |
| 卷号 | 26期号:9页码:1109-1117 |
| 关键词 | Poria cocos bioactive constituents LC-ESI-MSn rat metabolite |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.1756 |
| 文献子类 | Article |
| 英文摘要 | Poria cocos is a well-known medicinal plant widely used in China and other East Asian countries owing to its various therapeutic effects. However, the bioactive constituents responsible for the pharmacological effects of Poria cocos and their metabolites in vivo are still unclear to date. The aim of the present study was to develop a practical method based on the combined use of the liquid chromatography coupled with electrospray ionization multistage tandem mass spectrometry (LC-ESI-MSn) for the comprehensive and systematic separation and characterization of the bioactive constituents of Poria cocos extract and their metabolites in rats. Based on the proposed strategy, a total of 34 compounds were characterized from the extract of Poria cocos. Among them, eight were unambiguously identified by comparing their retention times and mass spectra with those of reference standards, and 26 were tentatively identified on the basis of their MSn fragmentation behaviors and molecular weight information from literatures. In vivo, seven compounds were successfully detected in rat urine whereas one was found in rat plasma. This study proposed a series of potential bioactive components and provided helpful chemical information for further research on the action mechanism of traditional Chinese medicine. Copyright (c) 2011 John Wiley & Sons, Ltd. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRIC DETECTION ; LANOSTANE-TYPE TRITERPENES ; CONSTITUENTS ; ACID ; STRATEGY ; ARRAY |
| 资助项目 | National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09301-001] ; National science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09501-030] ; National science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09103-373] ; Science and Technology Commission of Shanghai Municipality, China[09JC1416400] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000307220500011 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277971]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Bin |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Shanghai Inst Food & Drug Control, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ling, Yun,Chen, Mingcang,Wang, Ke,et al. Systematic screening and characterization of the major bioactive components of Poria cocos and their metabolites in rats by LC-ESI-MSn[J]. BIOMEDICAL CHROMATOGRAPHY,2012,26(9):1109-1117. |
| APA | Ling, Yun.,Chen, Mingcang.,Wang, Ke.,Sun, Zhaolin.,Li, Zhixiong.,...&Huang, Chenggang.(2012).Systematic screening and characterization of the major bioactive components of Poria cocos and their metabolites in rats by LC-ESI-MSn.BIOMEDICAL CHROMATOGRAPHY,26(9),1109-1117. |
| MLA | Ling, Yun,et al."Systematic screening and characterization of the major bioactive components of Poria cocos and their metabolites in rats by LC-ESI-MSn".BIOMEDICAL CHROMATOGRAPHY 26.9(2012):1109-1117. |
入库方式： OAI收割
来源：上海药物研究所
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