Simultaneous Determination of Four Sesquiterpenoids in Atractylodes Macrocephala Rhizoma by GC-FID: Optimisation of an Ultrasound-assisted Extraction by Central Composite Design
文献类型：期刊论文
| 作者 | Shi, Yang-Yang; Guan, Shu-Hong ; Tang, Ren-Neng; Tao, Si-Jia; Guo, De-An
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| 刊名 | PHYTOCHEMICAL ANALYSIS
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| 出版日期 | 2012-07 |
| 卷号 | 23期号:4页码:408-414 |
| 关键词 | Central composite design gas chromatography-flame ionisation detector quality control sesquiterpenoids Atractylodes Macrocephala Rhizoma |
| ISSN号 | 0958-0344 |
| DOI | 10.1002/pca.1373 |
| 文献子类 | Article |
| 英文摘要 | Introduction Atractylodes Macrocephala Rhizoma (AMR) is a traditional Chinese medicine containing several sesquiterpenoids with a series of effects. These bioactive compounds may be used as chemical markers for the quality control of AMR. It is necessary to optimise the extraction method and conditions in order to improve extraction productivity. Objective To develop a simple and effective method for the extraction of sesquiterpenoids from AMR and then to simultaneously determine four sesquiterpenoids, selina-4 (14), 7(11)-dien-8-one (SA), atractylenolide II (AII), atractylenolide III (AIII) and atractylenolide VII (AVII), in AMR. Methodology Ultrasound-assisted extraction (UAE) was optimised by central composite design (CCD) to obtain the maximum efficiency. The gas chromatography method was validated and applied for the quantification of four sesquiterpenoids. Results The optimum values of factors were: particle size (120?mesh), extraction time (26?min), extraction temperature (39 degrees C) and 31?mL of chloroform. The selectivity, linear range, limits of detection (LOD) and quantification (LOQ), accuracy, precision and repeatability of the method developed indicated its validity. The application of the method showed that the contents of four sesquiterpenoids in AMR were rather variable. Conclusion The results indicated that the described GC method could be used for the quality control of AMR and its related preparations. Meanwhile, this research revealed that UAE under optimum conditions could be considered as a powerful tool for the extraction of phytochemicals from plants. Copyright (C) 2011 John Wiley & Sons, Ltd. |
| WOS关键词 | ATRACTYLENOLIDE-III ; IN-VITRO ; OVATA ; VIVO |
| 资助项目 | National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09308-005] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09311001] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09502-020] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09304-002] ; Major Projects of Knowledge Innovation Program of the Chinese Academy of Sciences[KSCX2-YW-R-166] ; National Natural Science Foundation of China[81001630] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000305571500018 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278033]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Guo, De-An |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Yang-Yang,Guan, Shu-Hong,Tang, Ren-Neng,et al. Simultaneous Determination of Four Sesquiterpenoids in Atractylodes Macrocephala Rhizoma by GC-FID: Optimisation of an Ultrasound-assisted Extraction by Central Composite Design[J]. PHYTOCHEMICAL ANALYSIS,2012,23(4):408-414. |
| APA | Shi, Yang-Yang,Guan, Shu-Hong,Tang, Ren-Neng,Tao, Si-Jia,&Guo, De-An.(2012).Simultaneous Determination of Four Sesquiterpenoids in Atractylodes Macrocephala Rhizoma by GC-FID: Optimisation of an Ultrasound-assisted Extraction by Central Composite Design.PHYTOCHEMICAL ANALYSIS,23(4),408-414. |
| MLA | Shi, Yang-Yang,et al."Simultaneous Determination of Four Sesquiterpenoids in Atractylodes Macrocephala Rhizoma by GC-FID: Optimisation of an Ultrasound-assisted Extraction by Central Composite Design".PHYTOCHEMICAL ANALYSIS 23.4(2012):408-414. |
入库方式： OAI收割
来源：上海药物研究所
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