Proteomic studies on protective effects of salvianolic acids, notoginsengnosides and combination of salvianolic acids and notoginsengnosides against cardiac ischemic-reperfusion injury
文献类型：期刊论文
| 作者 | Yue, Qing-Xi; Xie, Fu-Bo; Song, Xiao-Yi; Wu, Wan-Ying ; Jiang, Bao-Hong; Guan, Shu-Hong; Yang, Min; Liu, Xuan; Guo, De-An
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| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
 |
| 出版日期 | 2012-06-01 |
| 卷号 | 141期号:2页码:659-667 |
| 关键词 | Danshen Sanqi Salvianolic acids Notoginsengnosides Ischemia-reperfusion Proteomics |
| ISSN号 | 0378-8741 |
| DOI | 10.1016/j.jep.2011.08.044 |
| 文献子类 | Article |
| 英文摘要 | Ethnopharmacological relevance: Salvia miltiorrhiza and Panax notoginseng are popularly used traditional Chinese medicine for cardiovascular disorders and they are often used in the form of combination. However, mechanisms of their cardioprotective effects were still not clear. In the present study, the protective effects of salvianolic acids (SA), notoginsengnosides (NG) and combination of SA and NG (CSN) against rat cardiac ischemia-reperfusion injury were checked and the protein expression profiles of heart tissues were examined to search their possible protein targets. Materials and methods: The cardioprotective effects of SA, NG and CSN were checked in a rat model of ischemia-reperfusion (IR) by temporarily occluding coronary artery for 20 min followed by reperfusion. Rats were grouped into sham-operation group, IR group, IR+SA group, IR+NC group and IR+CSN group. The plasma creatine kinase (CK) activities were measured using commercial kit and the percentages of infarcted area in total ventricle tissue were calculated after nitroblue-tetrazolium (N-BT) staining of heart tissue slices. Two-dimensional protein electrophoresis (2-DE) was used to check the protein expression profiles of heart tissues. Then, proteins differentially expressed between IR group and sham-operation group were identified using matrix assisted laser desorption ionization-time of flight-mass spectrometry/mass spectrometry (MALDI-TOF MS/MS). The regulative effects of SA, NG and CSN on these IR-related proteins were analyzed. Results: Treatments including SA, NG and CSN all showed cardioprotective effects against ischemia-reperfusion injury and CSN exhibited to be the best. Eighteen proteins involved in IR injury were found. These proteins are involved in pathways including energy metabolism, lipid metabolism, muscle contraction, heat shock stress, cell survival and proliferation. The regulation of these proteins by SA, NG or CSN suggested possible protein targets in their cardioprotective effects. Conclusions: SA and NG showed both similarity and difference in their protein targets involved in cardioprotective effects. The capability of CSN to regulate both protein targets of SA and NG might be the basis of CSN to show cardioprotective effects better than that of SA or NG. (C) 2011 Elsevier Ireland Ltd. All rights reserved. |
| WOS关键词 | PANAX-NOTOGINSENG ; MITOCHONDRIAL PROTEINS ; AQUEOUS EXTRACT ; MILTIORRHIZA ; INHIBITION ; MYOCARDIUM ; DANSHEN ; HEART ; PHARMACOLOGY ; MECHANISMS |
| 资助项目 | Chinese Academy of Sciences[KSCX2-YW-R-166] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09102-122] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09304-002] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09308-005] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09311-001] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09502-020] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000305201100018 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278049]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Liu, Xuan |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yue, Qing-Xi,Xie, Fu-Bo,Song, Xiao-Yi,et al. Proteomic studies on protective effects of salvianolic acids, notoginsengnosides and combination of salvianolic acids and notoginsengnosides against cardiac ischemic-reperfusion injury[J]. JOURNAL OF ETHNOPHARMACOLOGY,2012,141(2):659-667. |
| APA | Yue, Qing-Xi.,Xie, Fu-Bo.,Song, Xiao-Yi.,Wu, Wan-Ying.,Jiang, Bao-Hong.,...&Guo, De-An.(2012).Proteomic studies on protective effects of salvianolic acids, notoginsengnosides and combination of salvianolic acids and notoginsengnosides against cardiac ischemic-reperfusion injury.JOURNAL OF ETHNOPHARMACOLOGY,141(2),659-667. |
| MLA | Yue, Qing-Xi,et al."Proteomic studies on protective effects of salvianolic acids, notoginsengnosides and combination of salvianolic acids and notoginsengnosides against cardiac ischemic-reperfusion injury".JOURNAL OF ETHNOPHARMACOLOGY 141.2(2012):659-667. |
入库方式： OAI收割
来源：上海药物研究所
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