Metabolite Identification of Myricetin in Rats Using HPLC Coupled with ESI-MS
文献类型：期刊论文
| 作者 | Lin, Yun1,2; Wu, Bin1; Li, Zhixiong1 ; Hong, Ting2; Chen, Mingcang1; Tan, Yingzi2; Jiang, Jian2; Huang, Chenggang1
|
| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2012-06 |
| 卷号 | 75期号:11-12页码:655-660 |
| 关键词 | HPLC-ESI-MSn Myricetin Metabolites |
| ISSN号 | 0009-5893 |
| DOI | 10.1007/s10337-012-2239-z |
| 文献子类 | Article |
| 英文摘要 | Myricetin, a naturally occurring flavonol, shows multifarious pharmacological activities, e.g., antidiabetic, antioxidant, anti-inflammatory, antitumor, and liver protection effects. In order to obtain an understanding of the myricetin's metabolism in vivo, a rapid and sensitive method by high-performance liquid chromatography coupled with electrospray-ionization mass spectrometry (HPLC-MS (n) ) techniques was employed to investigate the biotransformation in rats after oral administration of myricetin. Recognition and structural exposition of the metabolites were operated by comparing the changes in molecular mass (Delta M) and MS (n) spectra with the parent drug. As a result, the parent compound and seven metabolites were found in rat plasma, urine, and feces. In addition, besides 3,5-dihydroxyphenylacetic acid (M1) and 3,4,5-trihydroxyphenylacetic acid (M2), five other compounds were first discovered in the metabolite research of myricetin. These results indicated that, besides ring-fission, there were methylate (M3, M4, M5) and glucuronide (M6, M7) biotransformations of myricetin occurring in vivo. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; CHROMATOGRAPHY ; DISEASE ; URINE |
| 资助项目 | National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09301-001] ; Science and Technology Commission of Shanghai Municipality[09DZ1971200] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000304557300010 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278059]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Huang, Chenggang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China; 2.Shanghai Univ Tradit Chinese Med, Dept Clin Pharmacol, Shuguang Hosp, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Yun,Wu, Bin,Li, Zhixiong,et al. Metabolite Identification of Myricetin in Rats Using HPLC Coupled with ESI-MS[J]. CHROMATOGRAPHIA,2012,75(11-12):655-660. |
| APA | Lin, Yun.,Wu, Bin.,Li, Zhixiong.,Hong, Ting.,Chen, Mingcang.,...&Huang, Chenggang.(2012).Metabolite Identification of Myricetin in Rats Using HPLC Coupled with ESI-MS.CHROMATOGRAPHIA,75(11-12),655-660. |
| MLA | Lin, Yun,et al."Metabolite Identification of Myricetin in Rats Using HPLC Coupled with ESI-MS".CHROMATOGRAPHIA 75.11-12(2012):655-660. |
入库方式： OAI收割
来源：上海药物研究所
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