Separation of Four Homoisoflavonoids from Caesalpinia sappan by High-speed Counter-current Chromatography
文献类型：期刊论文
| 作者 | Xu, Pingping; Guan, Shuhong ; Feng, Ruihong; Tang, Renneng; Guo, Dean
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| 刊名 | PHYTOCHEMICAL ANALYSIS
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| 出版日期 | 2012-05 |
| 卷号 | 23期号:3页码:228-231 |
| 关键词 | HSCCC brazilin 3-deoxysappanone B homoisoflavonoid Caesalpinia sappan |
| ISSN号 | 0958-0344 |
| DOI | 10.1002/pca.1347 |
| 文献子类 | Article |
| 英文摘要 | Introduction - The main chemical constituents of Caesalpinia sappan are homoisoflavonoids. Conventional column chromatographic techniques used for isolation of this type of compounds are tedious, time-consuming and waste solvents. High-speed counter-current chromatography (HSCCC) could be a suitable alternative for the enrichment and purification of these target compounds from traditional Chinese medicine (TCM). Objective - To establish a method to isolate four homoisoflavonoids in one-step separation from C. sappan by HSCCC. Methodology - The crude extract of C. sappan was fractionated by HSCCC using a two-phase solvent system consisting of chloroform-methanol-water (4:3:2, v/v/v). The separation conditions were:flow rate, 1.0mL/min; revolution speed, 900 rpm; detection wavelength, 280 nm; separation temperature, 25 degrees C; sample size, 120 mg crude sample dissolved in a mixture of the upper and lower phases (10 mL each). The retention of the stationary phase was 83%. Results - Five milligrams of 3'-deoxysappanol, 8 mg of 3-deoxysappanone B, 20 mg of 4-O-methylsappanol and 18 mg of brazilin were obtained in one-step separation from 120 mg of an ethyl acetate extracted fraction of C. sappan. Their purities were 99%, 97%, 90% and 85% by HPLC analysis. The mean recoveries of the four compounds were 83%, 86%, 93% and 85%, respectively. Conclusion - The study has shown that HSCCC is effective for the separation and enrichment of the target compounds at a large scale. Copyright (C) 2011 John Wiley & Sons, Ltd. |
| WOS关键词 | NITRIC-OXIDE SYNTHASE ; ABSOLUTE-CONFIGURATIONS ; PREPARATIVE ISOLATION ; 3 FLAVONOIDS ; BRAZILIN ; PURIFICATION ; CELLS ; L. |
| 资助项目 | National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09308-005] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09311-001] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09502-020] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09304-002] ; Chinese Academy of Sciences[KSCX2-YW-R-166] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000302292300006 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278105]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Guo, Dean |
| 作者单位 | Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Pingping,Guan, Shuhong,Feng, Ruihong,et al. Separation of Four Homoisoflavonoids from Caesalpinia sappan by High-speed Counter-current Chromatography[J]. PHYTOCHEMICAL ANALYSIS,2012,23(3):228-231. |
| APA | Xu, Pingping,Guan, Shuhong,Feng, Ruihong,Tang, Renneng,&Guo, Dean.(2012).Separation of Four Homoisoflavonoids from Caesalpinia sappan by High-speed Counter-current Chromatography.PHYTOCHEMICAL ANALYSIS,23(3),228-231. |
| MLA | Xu, Pingping,et al."Separation of Four Homoisoflavonoids from Caesalpinia sappan by High-speed Counter-current Chromatography".PHYTOCHEMICAL ANALYSIS 23.3(2012):228-231. |
入库方式： OAI收割
来源：上海药物研究所
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