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In vivo metabolism study of polygalic acid in rat using HPLC-ESI-MSn

文献类型：期刊论文

作者Ling, Yun1; Wu, Bin1; Wang, Ke2; Liu, Huihui1; Chen, Luying1; Fan, Mingsong1; Sun, Zhaolin1; Huang, Chenggang1
刊名BIOMEDICAL CHROMATOGRAPHY
出版日期2012-02
卷号26期号:2页码:220-224
关键词polygalic acid LC-ESI-MSn metabolite rat urine
ISSN号0269-3879
DOI10.1002/bmc.1650
文献子类Article
英文摘要A very simple and direct method has been established for the determination of polygalic acid and its metabolites in rat urine based on HPLC coupled with electrospray ionization multi-stage tandem mass spectrometry (HPLC-ESI-MSn). The rats were administered a single dose (100 mg/kg) of polygalic acid by oral gavage. The urine samples were collected and purified through a C18 solid-phase extraction cartridge, and then these pretreated samples were injected into a reversed-phase C18 column with a gradient elution program, whereas acetonitrile0.5% aqueous formic acid was used as mobile phase and detected by an on-line MS/MS system. As a result, the parent drug and its four metabolites were identified and characterized in rat urine for the first time by comparing their changes in molecular mass (M), retention times and full-scan MSn spectra with those of the parent drug. A possible metabolic pathway of polygalic acid was investigated and proposed. More importantly, the results demonstrated that the newly developed method (HPLC-ESI-MSn) was sensitive, simple and suitable for the determination of polygalic acid and its metabolites in biological samples. Copyright (C) 2011 John Wiley & Sons, Ltd.
WOS关键词MASS-SPECTROMETRY ; TENUIFOLIA ; MICE
资助项目National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09301-001] ; National science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09501-030] ; National science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09103-373] ; Science and Technology Commission of Shanghai Municipality, China[09JC1416400]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000298840300013
出版者WILEY-BLACKWELL
源URL[http://119.78.100.183/handle/2S10ELR8/278206]  
专题上海中药现代化研究中心
通讯作者Huang, Chenggang
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
2.Shanghai Inst Food & Drug Control, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Ling, Yun,Wu, Bin,Wang, Ke,et al. In vivo metabolism study of polygalic acid in rat using HPLC-ESI-MSn[J]. BIOMEDICAL CHROMATOGRAPHY,2012,26(2):220-224.
APA Ling, Yun.,Wu, Bin.,Wang, Ke.,Liu, Huihui.,Chen, Luying.,...&Huang, Chenggang.(2012).In vivo metabolism study of polygalic acid in rat using HPLC-ESI-MSn.BIOMEDICAL CHROMATOGRAPHY,26(2),220-224.
MLA Ling, Yun,et al."In vivo metabolism study of polygalic acid in rat using HPLC-ESI-MSn".BIOMEDICAL CHROMATOGRAPHY 26.2(2012):220-224.

入库方式： OAI收割

来源：上海药物研究所

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