Comparison of two officinal Chinese pharmacopoeia species of Ganoderma based on chemical research with multiple technologies and chemometrics analysis
文献类型：期刊论文
| 作者 | Da, Juan; Wu, Wan-Ying ; Hou, Jin-Jun; Long, Hua-Li; Yao, Shuai; Yang, Zhou; Cai, Lu-Ying; Yang, Min; Jiang, Bao-Hong; Liu, Xuan
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| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2012-01-27 |
| 卷号 | 1222页码:59-70 |
| 关键词 | Comparison Ganoderma lucidum Ganoderma sinense Polysaccharides Triterpenes |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2011.12.017 |
| 文献子类 | Article |
| 英文摘要 | Aim of the study: To investigate the chemical differences between Ganoderma lucidum (G. lucidum, Chizhi) and Ganoderma sinense (G. sinense. Zizhi). Materials and methods: Thirty two batches of commercial Ganoderma samples were collected, including 20 batches of G. lucidum and 12 batches of G. sinense cultivated in different geographical regions. Chemical substances in aqueous extract and alcoholic extract, mainly polysaccharides and triterpenes respectively, were investigated. Determination of polysaccharides was carried out with a high performance liquid chromatography with an variable wavelength detector. Meanwhile, analysis of triterpenes were performed on an ultraviolet spectrophotometer, an ultra performance liquid chromatography and a rapid resolution liquid chromatograph combined with an electrospray ionization mass spectrometer. Chromatograms and spectra for all batches and reference standards of main components were obtained and used for direct comparison. Further discussion was made on the basis of the result of principal component analysis (PCA). Results: Significant difference of triterpenes was shown between G. lucidum and G. sinense. In 20 batches of G. lucidum, 12 main components, including eight ganoderic acids and four ganoderenic acids were identified and ten of them were quantitatively determined, with the total content from 0.249% to 0.690%. However, none of those triterpenes was found in either batch of G. sinense. As for constituents of polysaccharides, seven monosaccharides were identified and four main components among them were quantitatively determined. Difference of polysaccharides was not directly observed, but latent information was revealed by PCA and the discrimination became feasible. Conclusions: G. lucidum and G. sinense were chemically different, which might result in pharmacological distinction. Preparations of traditional Chinese medicine (TCM) from Ganoderma should make accurate specification on the origin of species. (C) 2011 Elsevier B.V. All rights reserved. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; INHIBITORY-ACTIVITY ; QUALITY-CONTROL ; LUCIDUM ; TRITERPENOIDS ; ACID ; POLYSACCHARIDES ; FINGERPRINTS ; STEROLS ; SPORES |
| 资助项目 | United States Pharmacopoeia[00000000] ; Chinese Pharmacopoeia Commission[00000000] ; National S&T major special project for new innovative drug[2009ZX09308-005] ; National S&T major special project for new innovative drug[2009ZX09502-020] ; International Science and Technology Cooperation Project[2008DFA30350] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000300115700008 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278211]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Wan-Ying |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Da, Juan,Wu, Wan-Ying,Hou, Jin-Jun,et al. Comparison of two officinal Chinese pharmacopoeia species of Ganoderma based on chemical research with multiple technologies and chemometrics analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1222:59-70. |
| APA | Da, Juan.,Wu, Wan-Ying.,Hou, Jin-Jun.,Long, Hua-Li.,Yao, Shuai.,...&Guo, De-An.(2012).Comparison of two officinal Chinese pharmacopoeia species of Ganoderma based on chemical research with multiple technologies and chemometrics analysis.JOURNAL OF CHROMATOGRAPHY A,1222,59-70. |
| MLA | Da, Juan,et al."Comparison of two officinal Chinese pharmacopoeia species of Ganoderma based on chemical research with multiple technologies and chemometrics analysis".JOURNAL OF CHROMATOGRAPHY A 1222(2012):59-70. |
入库方式： OAI收割
来源：上海药物研究所
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