中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 上海中药现代化研究中心
Isolation, identification and antiviral activities of metabolites of calycosin-7-O-beta-D-glucopyranoside

文献类型：期刊论文

作者Chen, Luying1; Li, Zhixiong1; Tang, Yihong1; Cui, Xiaolan3; Luo, Ronghua2; Guo, Shanshan3; Zheng, Yongtang2; Huang, Chenggang1
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
出版日期2011-09-10
卷号56期号:2页码:382-389
关键词Calycosin-7-O-beta-D-glucopyranoside Metabolites Antiviral activity
ISSN号0731-7085
DOI10.1016/j.jpba.2011.05.033
文献子类Article
英文摘要In vivo and in vitro metabolites of calycosin-7-O-beta-D-glucopyranoside in rats were identified using a specific and sensitive high performance liquid chromatography-tandem mass spectrometry (HPLC-MS(n)) method. The parent compound and twelve metabolites were found in rat urine after oral administration of calycosin-7-O-beta-D-glucopyranoside. The parent compound and six metabolites were detected in rat plasma. In heart, liver, spleen, lung and kidney samples, respectively, six, eight, seven, nine and nine metabolites were identified, in addition to the parent compound. Three metabolites, but no trace of parent drug, were found in the rat intestinal flora incubation mixture and feces, which demonstrated cleavage of the glycosidic bond of the parent compound in intestines. The main phase I metabolic pathways of calycosin-7-O-beta-D-glucopyranoside in rats were deglycosylation, dehydroxylation and demethylation reactions; phase II metabolism included sulfation, methylation, glucuronidation and glycosylation (probably). Furthermore, two metabolites commonly found in rat urine, plasma and tissues were isolated from feces and characterized by NMR. The antiviral activities of the metabolite calycosin against coxsackie virus B(3) (CVB(3)) and human immunodeficiency virus (HIV) were remarkably stronger than those of calycosin-7-O-beta-D-glucopyranoside. (C) 2011 Elsevier B.V. All rights reserved.
WOS关键词IN-VITRO ; MASS-SPECTROMETRY ; RAT PLASMA ; VIVO ; FRAGMENTATION ; MONGHOLICUS ; GLYCOSIDES ; URINE ; HPLC
资助项目National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09301-001] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09308-005] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09501-030] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09103-334] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09301-005-007] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09501-029]
WOS研究方向Chemistry ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000292785900028
出版者ELSEVIER SCIENCE BV
源URL[http://119.78.100.183/handle/2S10ELR8/278403]  
专题上海中药现代化研究中心
通讯作者Huang, Chenggang
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
2.Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Kunming 650223, Peoples R China;
3.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Luying,Li, Zhixiong,Tang, Yihong,et al. Isolation, identification and antiviral activities of metabolites of calycosin-7-O-beta-D-glucopyranoside[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2011,56(2):382-389.
APA Chen, Luying.,Li, Zhixiong.,Tang, Yihong.,Cui, Xiaolan.,Luo, Ronghua.,...&Huang, Chenggang.(2011).Isolation, identification and antiviral activities of metabolites of calycosin-7-O-beta-D-glucopyranoside.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,56(2),382-389.
MLA Chen, Luying,et al."Isolation, identification and antiviral activities of metabolites of calycosin-7-O-beta-D-glucopyranoside".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 56.2(2011):382-389.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。