Liquid Chromatography Tandem Mass Spectrometry Detection of Ablmosechus manihot and Its Major Metabolites in Rat Urine After Oral Administration
文献类型：期刊论文
| 作者 | Jiang, Weixing1; Liu, Shuang1,2; Wu, Bin2; Huang, Chenggang2
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| 刊名 | ASIAN JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2011-04 |
| 卷号 | 23期号:4页码:1643-1647 |
| 关键词 | Ablmosechus manihot HPLC-MS(n) Metabolites Urine |
| ISSN号 | 0970-7077 |
| 文献子类 | Article |
| 英文摘要 | A highly specific and sensitive method using high performance liquid chromatography-electrospray ionization (ESI) tandem ion trap mass spectrometry(HPLC-ESI-MS(n)) was developed to study on A. manihot and its metabolites in rat urine. The gradient elution reversed phase HPLC system using water of 0.5 % formic acid and acetonitrile as gradient Solvent mixtures was able to separate all metabolites of Ablmosechus manihot. The A. manihot were negative identified by the protonated molecules [M-H](-) and further characterized by tandem mass spectrometric analysis with each it giving a characteristic collision induced dissociation product ion spectrum. Furthermore, 2 prototype constituents and 7 metabolites in urine were detected and elucidated. This convenient HPLC-MS(n) method could be used to identify the chemical components of extract of A. manihot as well as their metabolites and to reveal their possible metabolic mechanism of action in vivo. |
| WOS关键词 | ABELMOSCHUS-MANIHOT ; ARRAY DETECTION ; HPLC ; FLAVONOLS ; FLOWERS ; EXTRACT ; PLASMA |
| 资助项目 | National Science & Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09301-001] ; National Science & Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09308-005] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000288465100053 |
| 出版者 | ASIAN JOURNAL OF CHEMISTRY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278572]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Bin |
| 作者单位 | 1.Haerbin Univ Commerce, Haerbin 150076, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Weixing,Liu, Shuang,Wu, Bin,et al. Liquid Chromatography Tandem Mass Spectrometry Detection of Ablmosechus manihot and Its Major Metabolites in Rat Urine After Oral Administration[J]. ASIAN JOURNAL OF CHEMISTRY,2011,23(4):1643-1647. |
| APA | Jiang, Weixing,Liu, Shuang,Wu, Bin,&Huang, Chenggang.(2011).Liquid Chromatography Tandem Mass Spectrometry Detection of Ablmosechus manihot and Its Major Metabolites in Rat Urine After Oral Administration.ASIAN JOURNAL OF CHEMISTRY,23(4),1643-1647. |
| MLA | Jiang, Weixing,et al."Liquid Chromatography Tandem Mass Spectrometry Detection of Ablmosechus manihot and Its Major Metabolites in Rat Urine After Oral Administration".ASIAN JOURNAL OF CHEMISTRY 23.4(2011):1643-1647. |
入库方式： OAI收割
来源：上海药物研究所
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