Characterization of the Multiple Absorbed Constituents in Rats after Oral Administration of Chai-Huang Decoction by Liquid Chromatography Coupled with Electrospray-Ionization Mass Spectrometry
文献类型：期刊论文
| 作者 | Ye, Guan2; Tang, Yi-Hong2; Wang, Gui-Yun1; Li, Zhi-Xiong2 ; Zhu, Hai-Yan2; Ma, Chun-Hui2; Sun, Zhao-Ling2; Huang, Cheng-Gang2
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| 刊名 | CHEMISTRY & BIODIVERSITY
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| 出版日期 | 2010-12 |
| 卷号 | 7期号:12页码:2917-2930 |
| ISSN号 | 1612-1872 |
| DOI | 10.1002/cbdv.200900387 |
| 文献子类 | Article |
| 英文摘要 | A rapid, sensitive, and specific method by high-performance liquid chromatography (HPLC) coupled to diode-array detection (DAD) and tandem mass spectrometry (MS) techniques was developed for the identification of absorbed constituents and their metabolites in rats after the oral administration of a Chai-Huang decoction (CHD), which consists of Bupleurum chinense and Scutellaria baicalensis in the proportion 1:1 (w/w). By comparing their retention times and MS data with those of authentic compounds and published data, a total of 14 compounds were identified in the CHD samples. In addition, eleven and seven compounds were characterized in the urine and serum samples of the rats, respectively. The results indicated that the main absorbed constituents were chrysin-6-C-arabinosyl-8-C-glucoside, chrysin-6-C-glucosyl-8-C-arabinoside, baicalin, wogonin 5-O-glucoside, oroxylin A-7-O-glucuronide, wogonoside, saikosaponin A, saikosaponin C, saikosaponin D, baicalein, and wogonin. These compounds might be responsible for the curative effects of the CHD. The findings demonstrated that the proposed method could be used to rapidly and simultaneously analyze and screen the multiple absorbed bioactive constituents in a formula of traditional Chinese medicines (TCM). This is very important not only for the pharmaceutical discovery process and the quality control of crude drugs but also to explain the mechanisms of action of TCM. |
| WOS关键词 | SCUTELLARIA-BAICALENSIS-GEORGI ; LC-MS/MS ; MAJOR METABOLITES ; MEDICINAL HERBS ; RADIX-BUPLEURI ; BAICALIN ; IDENTIFICATION ; WOGONIN ; EXTRACT ; URINE |
| 资助项目 | Science and Technology Commission of the Shanghai Municipality[02DZ19145] ; National Science & Technology Major Project[2009ZX09301-001] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000285393800011 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278702]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Huang, Cheng-Gang |
| 作者单位 | 1.Anshan Normal Univ, Anshan Hlth Sch, Anshan 114001, Liaoning Prov, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Ye, Guan,Tang, Yi-Hong,Wang, Gui-Yun,et al. Characterization of the Multiple Absorbed Constituents in Rats after Oral Administration of Chai-Huang Decoction by Liquid Chromatography Coupled with Electrospray-Ionization Mass Spectrometry[J]. CHEMISTRY & BIODIVERSITY,2010,7(12):2917-2930. |
| APA | Ye, Guan.,Tang, Yi-Hong.,Wang, Gui-Yun.,Li, Zhi-Xiong.,Zhu, Hai-Yan.,...&Huang, Cheng-Gang.(2010).Characterization of the Multiple Absorbed Constituents in Rats after Oral Administration of Chai-Huang Decoction by Liquid Chromatography Coupled with Electrospray-Ionization Mass Spectrometry.CHEMISTRY & BIODIVERSITY,7(12),2917-2930. |
| MLA | Ye, Guan,et al."Characterization of the Multiple Absorbed Constituents in Rats after Oral Administration of Chai-Huang Decoction by Liquid Chromatography Coupled with Electrospray-Ionization Mass Spectrometry".CHEMISTRY & BIODIVERSITY 7.12(2010):2917-2930. |
入库方式： OAI收割
来源：上海药物研究所
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