中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 上海中药现代化研究中心
Cytotoxicity of 9,11-Dehydroergosterol Peroxide Isolated from Ganoderma lucidum and its Target-related Proteins

文献类型：期刊论文

作者Cui, Ya-Jun2; Guan, Shu-Hong1; Feng, Li-Xing1; Song, Xiao-Yi2; Ma, Chao1; Cheng, Chun-Ru1; Wang, Wen-Bo1; Wu, Wan-Ying1; Yue, Qing-Xi1; Liu, Xuan1
刊名NATURAL PRODUCT COMMUNICATIONS
出版日期2010-08
卷号5期号:8页码:1183-1186
关键词9,11-dehydroergosterol peroxide Ganoderma lucidum cytotoxicity proteomic stathmin 1
ISSN号1934-578X
文献子类Article
英文摘要The cytotoxicty of 9,11-dehydroergosterol peroxide (DHEP) isolated from the fruiting bodies of Ganoderma lucidum on HeLa cells was studied. DHEP treatment for 48 h inhibited the proliferation of HeLa human cervical carcinoma cells with an IC50-value of 8.58 +/- 0.98 mu M. Morphological changes of DHEP-treated cells indicated that DHEP induced apoptosis in HeLa cells. To identify the cellular targets of DHEP, two-dimensional electrophoresis analysis was performed to compare the protein expression profiles of DHEP-treated cells with that of control cells. Proteins altered in expressional level after DHEP exposure were identified by MALDI-TOF MS/MS. The cytotoxic effect of DHEP was associated with regulated expression of 6 proteins. Stathmin 1 might be an important target-related protein of DHEP. The regulation of stathmin 1 by DHEP treatment was also confirmed by Western blotting.
WOS关键词CELL-CYCLE ARREST ; LANOSTANOID TRITERPENES ; MECHANISM
资助项目National Natural Science Foundation of China[30701077] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09102-122] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09308-005] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program", China[2009ZX09502-020]
WOS研究方向Pharmacology & Pharmacy ; Food Science & Technology
语种英语
WOS记录号WOS:000281074200006
出版者NATURAL PRODUCTS INC
源URL[http://119.78.100.183/handle/2S10ELR8/278821]  
专题上海中药现代化研究中心
通讯作者Liu, Xuan
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
2.Shanghai Univ Chinese Tradit Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Cui, Ya-Jun,Guan, Shu-Hong,Feng, Li-Xing,et al. Cytotoxicity of 9,11-Dehydroergosterol Peroxide Isolated from Ganoderma lucidum and its Target-related Proteins[J]. NATURAL PRODUCT COMMUNICATIONS,2010,5(8):1183-1186.
APA Cui, Ya-Jun.,Guan, Shu-Hong.,Feng, Li-Xing.,Song, Xiao-Yi.,Ma, Chao.,...&Guo, De-An.(2010).Cytotoxicity of 9,11-Dehydroergosterol Peroxide Isolated from Ganoderma lucidum and its Target-related Proteins.NATURAL PRODUCT COMMUNICATIONS,5(8),1183-1186.
MLA Cui, Ya-Jun,et al."Cytotoxicity of 9,11-Dehydroergosterol Peroxide Isolated from Ganoderma lucidum and its Target-related Proteins".NATURAL PRODUCT COMMUNICATIONS 5.8(2010):1183-1186.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。