Effects of alpha-Asarone on the Glutamate Transporter EAAC1 in Xenopus Oocytes
文献类型：期刊论文
| 作者 | Gu, Quanbao; Du, Huiming; Ma, Chunhui; Fotis, Heike; Wu, Bin; Huang, Chenggang3 ; Schwarz, Wolfgang1,2
|
| 刊名 | PLANTA MEDICA
 |
| 出版日期 | 2010-04 |
| 卷号 | 76期号:6页码:595-598 |
| 关键词 | Acorus tatarinowii Acoraceae alpha-asarone neurotransmitter transporter glutamate Xenopus oocytes voltage clamp |
| ISSN号 | 0032-0943 |
| DOI | 10.1055/s-0029-1240613 |
| 文献子类 | Article |
| 英文摘要 | The major excitatory neurotransmitter transporter EAAC1 in the mammalian central nervous system is considered a possible target for Chinese herbal medicine. Extracts of Acorus tatarinowii (Schott) were tested for their effects on EAAC1 activity. Xenopus oocytes with heterologously expressed EAAC1 were used as the model system. Rate of glutamate uptake was determined by means of the isotopic tracer technique. Glutamate-induced current was recorded under a two-electrode voltage clamp. As a highly effective component, a-asarone was identified. The rate of glutamate uptake was stimulated by 200 mu M of alpha-asarone by about 15%. In contrast, the same concentration reduced the EAAC1-mediated current by about 35% at a holding potential of - 60 mV; half maximum inhibition was obtained at about 60 mu M. Our experimental data suggest that both stimulation of glutamate uptake and inhibition of EAAC1-mediated current by alpha-asarone could contribute to reduced excitatory activity. |
| WOS关键词 | EXPRESSION ; CHANNEL |
| 资助项目 | Science Foundation of the Shanghai Municipal Commission of Science and Technology[05DZ19747] ; National Basic Research Program of China[2005CB523306] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000277658800007 |
| 出版者 | GEORG THIEME VERLAG KG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278938]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Schwarz, Wolfgang |
| 作者单位 | 1.Max Planck Inst Biophys, D-6000 Frankfurt, Germany; 2.Shanghai Res Ctr Acupuncture & Meridians, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Quanbao,Du, Huiming,Ma, Chunhui,et al. Effects of alpha-Asarone on the Glutamate Transporter EAAC1 in Xenopus Oocytes[J]. PLANTA MEDICA,2010,76(6):595-598. |
| APA | Gu, Quanbao.,Du, Huiming.,Ma, Chunhui.,Fotis, Heike.,Wu, Bin.,...&Schwarz, Wolfgang.(2010).Effects of alpha-Asarone on the Glutamate Transporter EAAC1 in Xenopus Oocytes.PLANTA MEDICA,76(6),595-598. |
| MLA | Gu, Quanbao,et al."Effects of alpha-Asarone on the Glutamate Transporter EAAC1 in Xenopus Oocytes".PLANTA MEDICA 76.6(2010):595-598. |
入库方式： OAI收割
来源：上海药物研究所
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