Proteomic analysis of possible target-related proteins of cyclophosphamide in mice thymus
文献类型：期刊论文
| 作者 | Ma, Chao; Yue, Qing-Xi; Guan, Shu-Hong ; Wu, Wan-Ying; Yang, Min; Jiang, Bao-Hong; Liu, Xuan; Guo, De-An
|
| 刊名 | FOOD AND CHEMICAL TOXICOLOGY
 |
| 出版日期 | 2009-08 |
| 卷号 | 47期号:8页码:1841-1847 |
| 关键词 | Cyclophosphamide Ganoderma lucidum Polysaccharides Thymus Immuno-suppression Proteomics |
| ISSN号 | 0278-6915 |
| DOI | 10.1016/j.fct.2009.04.041 |
| 文献子类 | Article |
| 英文摘要 | Cyclophosphamide (Cy) could induce immuno-suppression such as thymus atrophy and inhibition of lymphocyte proliferation response in mice. But, the mechanism of its effect is still not clear. Polysaccharides isolated from spore of Ganoderma lucidum (GL-SP) could, at least partly, restore the immunological effects against Cy-induced immuno-suppression. In the present study, a two-dimensional gel electrophoresis (2-DE)-based comparative proteomic method was conducted to identify the possible target-related proteins of Cy in mice thymus including those could be influenced by GL-SP. Fifteen proteins differentially expressed in Cy-treated mice compared with control were identified. Among the fifteen proteins, combined use of GL-SP with Cy could not affect Cy-induced expression change of three proteins, totally prevented Cy-induced expression change of six proteins, partly prevented Cy-induced expression change of four proteins and further enhanced Cy-induced expression change of two proteins. Results of the present study shed light on the mechanism of Cy-induced immuno-suppression as well as the protective effect of GL-SP against toxicity of Cy. (C) 2009 Elsevier Ltd. All rights reserved. |
| WOS关键词 | OXIDOREDUCTASE COMPLEX-I ; GANODERMA-LUCIDUM ; T-CELLS ; EXPRESSION ; APOPTOSIS ; POLYSACCHARIDES ; LYMPHOCYTES ; METABOLISM ; MUTATIONS ; MECHANISM |
| 资助项目 | National Natural Science Foundation of China[30701077] ; Science and Technology Commission of Shanghai Municipality[06DZ19731] ; Shanghai Pudong Science and Technology Committee[PKJ2006-L07] |
| WOS研究方向 | Food Science & Technology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000269100700018 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279176]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Liu, Xuan |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Chao,Yue, Qing-Xi,Guan, Shu-Hong,et al. Proteomic analysis of possible target-related proteins of cyclophosphamide in mice thymus[J]. FOOD AND CHEMICAL TOXICOLOGY,2009,47(8):1841-1847. |
| APA | Ma, Chao.,Yue, Qing-Xi.,Guan, Shu-Hong.,Wu, Wan-Ying.,Yang, Min.,...&Guo, De-An.(2009).Proteomic analysis of possible target-related proteins of cyclophosphamide in mice thymus.FOOD AND CHEMICAL TOXICOLOGY,47(8),1841-1847. |
| MLA | Ma, Chao,et al."Proteomic analysis of possible target-related proteins of cyclophosphamide in mice thymus".FOOD AND CHEMICAL TOXICOLOGY 47.8(2009):1841-1847. |
入库方式： OAI收割
来源：上海药物研究所
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