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Simultaneous Quantification of Eight Major Bioactive Phenolic Compounds in Chinese Propolis by High-Performance Liquid Chromatography

文献类型：期刊论文

作者Sha, Na1,2; Huang, Hui-Lian1,2; Zhang, Jin-Qiang2; Chen, Guang-Tong2; Tao, Si-Jia2; Yang, Min2; Li, Xing-Nuo2; Li, Ping1; Guo, De-An2
刊名NATURAL PRODUCT COMMUNICATIONS
出版日期2009-06
卷号4期号:6页码:813-818
关键词Propolis phenolic compounds R-P-HPLC quality control
ISSN号1934-578X
文献子类Article
英文摘要A simple, sensitive and specific high-performance liquid chromatography-UV (HPLC-UV) method has been developed to simultaneously quantify the eight major bioactive phenolic compounds in Chinese propolis, namely caffeic acid, isoferulic acid, 3,4-dimethoxycinnamic acid, pinobanksin 5-methyl ether, pinocembrin, benzyl caffeate, chrysin and galangin. This HPLC assay was performed on an Agilent Zorbax Extend-C-18 (250 x 4.6 mm, 5 mu m) column with a gradient of methanol and 0.2% aqueous acetic acid (v/v) in 50 min, at a flow rate of 1.0 mL/min, and detected at 290 rim. All calibration curves showed good linearity (r(2)>0.999) within the test ranges. The intra- and inter-day assay precision (RSD) of eight phenolic compounds were in the range of 0.07-4.92%. The recoveries were between 98.3% and 104.8%. This assay was applied to the evaluation of nineteen samples from different origins in China. The results indicated that the developed assay could be readily utilized for the quality control of propolis.
WOS关键词SALVIA-MILTIORRHIZA ; MEDICINAL PREPARATIONS ; BRAZILIAN PROPOLIS ; MASS-SPECTROMETRY ; PLANT-ORIGIN ; DAD METHOD ; HPLC-DAD ; CONSTITUENTS ; ANTIFUNGAL ; ROOTS
资助项目Ministry of Science and Technology of China[2006BAI08B03-03] ; Shanghai Commission of Science and Technology[00000000]
WOS研究方向Pharmacology & Pharmacy ; Food Science & Technology
语种英语
WOS记录号WOS:000267313900015
出版者NATURAL PRODUCTS INC
源URL[http://119.78.100.183/handle/2S10ELR8/279231]  
专题上海中药现代化研究中心
通讯作者Guo, De-An
作者单位1.China Pharmaceut Univ, Coll Tradit Chinese Mat Med, Nanjing 210009, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China;
推荐引用方式
GB/T 7714		 Sha, Na,Huang, Hui-Lian,Zhang, Jin-Qiang,et al. Simultaneous Quantification of Eight Major Bioactive Phenolic Compounds in Chinese Propolis by High-Performance Liquid Chromatography[J]. NATURAL PRODUCT COMMUNICATIONS,2009,4(6):813-818.
APA Sha, Na.,Huang, Hui-Lian.,Zhang, Jin-Qiang.,Chen, Guang-Tong.,Tao, Si-Jia.,...&Guo, De-An.(2009).Simultaneous Quantification of Eight Major Bioactive Phenolic Compounds in Chinese Propolis by High-Performance Liquid Chromatography.NATURAL PRODUCT COMMUNICATIONS,4(6),813-818.
MLA Sha, Na,et al."Simultaneous Quantification of Eight Major Bioactive Phenolic Compounds in Chinese Propolis by High-Performance Liquid Chromatography".NATURAL PRODUCT COMMUNICATIONS 4.6(2009):813-818.

入库方式： OAI收割

来源：上海药物研究所

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