Mitogen-activated protein kinase (MAPK) pathway mediates the oestrogen-like activities of ginsenoside Rg1 in human breast cancer (MCF-7) cells
文献类型：期刊论文
| 作者 | Lau, Wai-Sum2,4; Chen, Wen-Fang3,4; Chan, Robbie Yat-Kan2,4; Guo, De-An1 ; Wong, Man-Sau2,4
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| 刊名 | BRITISH JOURNAL OF PHARMACOLOGY
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| 出版日期 | 2009-04 |
| 卷号 | 156期号:7页码:1136-1146 |
| 关键词 | MAPK ER alpha phytoestrogens ginsenoside Rg1 |
| ISSN号 | 0007-1188 |
| DOI | 10.1111/j.1476-5381.2009.00123.x |
| 文献子类 | Article |
| 英文摘要 | The present study was designed to determine how ginsenoside Rg1, an active ingredient in ginseng root, exerts its oestrogenic effects. We hypothesize that Rg1 may exert oestrogen-like actions in MCF-7 cells by activating the mitogen-activated protein kinase (MAPK) pathway in a ligand-independent manner. MCF-7 cells were co-incubated with the MAPK inhibitor PD98059 to determine whether the stimulant effects of Rg1 on cell proliferation, the induction of IGF-IR and pS2, the functional transactivation of oestrogen receptor-alpha (ER alpha), as well as ER alpha phosphorylation are dependent on MAPK. The time-dependent responses of mitogen-activated protein kinase kinase (MEK) and extracellular signal-regulated protein kinase (ERK) to Rg1 in MCF-7 cells were studied. The responses of MEK phosphorylation to Rg1 in oestrogen receptor (ER)-negative HEK293 cells were also determined. The effects of Rg1 on cell proliferation and IGF-IR protein expression were studied in the presence of tyrosine kinase inhibitor genistein to elucidate the involvement of tyrosine kinase in mediating these effects. The oestrogenic effects of Rg1 in MCF-7 cells were abolished in the presence of PD98059. Rg1 could induce MEK protein expression and the phosphorylation level of MEK and ERK significantly in a time- and dose-dependent manner. Rg1 activated MEK phosphorylation in ER-negative HEK293 cells in a time- and dose-dependent manner. Rg1 induction of cell proliferation and IGF-IR protein expression was abolished by co-treatment with genistein. Taken together, these results show that the MAPK pathway is involved in mediating the oestrogen-like actions of Rg1 in MCF-7 cells and suggest that Rg1 may activate ER alpha via MEK/ERK in a ligand-independent manner. |
| WOS关键词 | FACTOR-I RECEPTOR ; LIGAND-INDEPENDENT ACTIVATION ; GROWTH-FACTOR RECEPTOR ; DIETARY PHYTOESTROGENS ; GENISTEIN ; ALPHA ; PHOSPHORYLATION ; MECHANISMS |
| 资助项目 | Hong Kong Special Administrative Region, China[AOE/P-10/01] ; RGC General Research Fund[5636/07M] ; The Hong Kong Polytechnic University[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000264783300012 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279270]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wong, Man-Sau |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai Res Ctr TCM Modernizat, Shanghai 200031, Peoples R China 2.State Key Lab Chinese Med & Mol Pharmacol, Shenzhen, Peoples R China; 3.Qingdao Univ, Coll Med, Dept Physiol, Qingdao 266071, Peoples R China; 4.Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Inst Mol Technol Drug Discovery & Synth, Cent Lab, Kowloon, Hong Kong, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Lau, Wai-Sum,Chen, Wen-Fang,Chan, Robbie Yat-Kan,et al. Mitogen-activated protein kinase (MAPK) pathway mediates the oestrogen-like activities of ginsenoside Rg1 in human breast cancer (MCF-7) cells[J]. BRITISH JOURNAL OF PHARMACOLOGY,2009,156(7):1136-1146. |
| APA | Lau, Wai-Sum,Chen, Wen-Fang,Chan, Robbie Yat-Kan,Guo, De-An,&Wong, Man-Sau.(2009).Mitogen-activated protein kinase (MAPK) pathway mediates the oestrogen-like activities of ginsenoside Rg1 in human breast cancer (MCF-7) cells.BRITISH JOURNAL OF PHARMACOLOGY,156(7),1136-1146. |
| MLA | Lau, Wai-Sum,et al."Mitogen-activated protein kinase (MAPK) pathway mediates the oestrogen-like activities of ginsenoside Rg1 in human breast cancer (MCF-7) cells".BRITISH JOURNAL OF PHARMACOLOGY 156.7(2009):1136-1146. |
入库方式： OAI收割
来源：上海药物研究所
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