Systematic profiling and comparison of the lipidomes from Panax ginseng, P. quinquefolius, and P. notoginseng by ultrahigh performance supercritical fluid chromatography/high-resolution mass spectrometry and ion mobility-derived collision cross section measurement
文献类型：期刊论文
| 作者 | Shi, Xiaojian; Yang, Wenzhi; Qiu, Shi; Hou, Jinjun ; Wu, Wanying; Guo, Dean
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| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2018-05-04 |
| 卷号 | 1548页码:64-75 |
| 关键词 | Lipidomics Ultrahigh performance supercritical fluid chromatography Ion mobility spectroscopy Collision cross section Panax Quality control |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2018.03.025 |
| 文献子类 | Article |
| 英文摘要 | Lipidomics currently is still confronted with challenges from chromatographic separation and lipids identification. Here we report a lipidomics platform by integrating ultrahigh performance supercritical fluid chromatography/quadrupole time-of-flight mass spectrometry (UHPSFC/QTOF-MS) and collision cross section (CCS) measurement using ion mobility spectroscopy/time-of-flight mass spectrometry (IMS/QTOF-MS), aiming to enhance the profiling performance and identification reliability of lipids. The lipidomes extracted from three congeneric Panax species (P. ginseng, P. quinquefolius, and P. notoginseng) by methyl tert-butyl ether are comprehensively profiled and compared by use of this platform. A potent UHPSFC/QTOF-MS approach was developed on a 1.7-mu m particles packed Torus 2-PIC column using CH3OH (in CO2) as a modifier and CH3OH/0.2 mM ammonium acetate as the makeup liquid, enabling well resolution of six lipid subclasses by both positive and negative MSE modes. In contrast to the reversed-phase chromatography, "normal-phase" like elution order and better resolution of polar lipids and some lipid isomers were achieved by UHPSFC separation. Pattern recognition chemometric analysis of 60 batches of Ginseng samples ultimately unveiled 24 lipid markers, of which triacylglycerols were the most important. Aside from the automated MS database searching against HMDB and LIPID MAPS, the application of CCS retrieval or CCS prediction improved lipid identification by reducing the possible hits. In conclusion, this integral platform can significantly improve the chromatographic separation and the reliability of lipids identification in lipidomics studies. It is the first report that systematically compares the lipidomic difference of three reputable Panax species, providing useful information for their quality control in addition to ginsenoside analysis. (C) 2018 Elsevier B.V. All rights reserved. |
| WOS关键词 | EFFICIENT DISCOVERY ; SHOTGUN LIPIDOMICS ; NATURAL COMPOUNDS ; METABOLOMICS ; IDENTIFICATION ; GINSENOSIDES ; DIFFERENTIATION ; PREDICTION ; DIVERSITY ; MEDICINE |
| 资助项目 | National Natural Science Foundation of China[81503240] ; National Natural Science Foundation of China[81473344] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000430779500008 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279769]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Wanying; Guo, Dean |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Haike Rd 501, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Xiaojian,Yang, Wenzhi,Qiu, Shi,et al. Systematic profiling and comparison of the lipidomes from Panax ginseng, P. quinquefolius, and P. notoginseng by ultrahigh performance supercritical fluid chromatography/high-resolution mass spectrometry and ion mobility-derived collision cross section measurement[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1548:64-75. |
| APA | Shi, Xiaojian,Yang, Wenzhi,Qiu, Shi,Hou, Jinjun,Wu, Wanying,&Guo, Dean.(2018).Systematic profiling and comparison of the lipidomes from Panax ginseng, P. quinquefolius, and P. notoginseng by ultrahigh performance supercritical fluid chromatography/high-resolution mass spectrometry and ion mobility-derived collision cross section measurement.JOURNAL OF CHROMATOGRAPHY A,1548,64-75. |
| MLA | Shi, Xiaojian,et al."Systematic profiling and comparison of the lipidomes from Panax ginseng, P. quinquefolius, and P. notoginseng by ultrahigh performance supercritical fluid chromatography/high-resolution mass spectrometry and ion mobility-derived collision cross section measurement".JOURNAL OF CHROMATOGRAPHY A 1548(2018):64-75. |
入库方式： OAI收割
来源：上海药物研究所
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