Characteristic Chromatogram: A Method of Discriminate and Quantitative Analysis for Quality Evaluation of Uncaria Stem with Hooks
文献类型：期刊论文
| 作者 | Hou, Jinjun1 ; Feng, Ruihong1; Zhang, Yibei1; Pan, Huiqin1; Yao, Shuai1; Han, Sumei1; Feng, Zijin2; Cai, Luying1; Wu, Wanying1; Guo, De-an1
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| 刊名 | PLANTA MEDICA
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| 出版日期 | 2018-04 |
| 卷号 | 84期号:6-7页码:449-456 |
| 关键词 | Uncaria rhynchophylla Rubiaceae isorhynchophylline European pharmacopeia multiple botanical origins quality control |
| ISSN号 | 0032-0943 |
| DOI | 10.1055/s-0043-123827 |
| 文献子类 | Article |
| 英文摘要 | It remains a challenge to establish new monographs for herbal drugs derived from multiple botanical sources. Specifically, the difficulty involves discriminating and quantifying these herbs with components whose levels vary markedly among different samples. Using Uncaria stem with hooks as an example, a characteristic chromatogram was proposed to discriminate its five botanical origins and to quantify its characteristic components in the chromatogram. The characteristic chromatogram with respect to the components of Uncaria stem with hooks with the five botanical origins was established using 0.02% diethylamine and acetonitrile as the mobile phase. The total analysis time was 50min and the detection wavelength was 245nm. Using the same chromatogram parameters, the single standard to determine multicomponents method was validated to simultaneously quantify nine indole alkaloids, including vincosamide, 3-dihydrocadambine, isocorynoxeine, corynoxeine, isorhynchophylline, rhynchophylline, hirsuteine, hirsutine, and geissoschizine methyl ether. The results showed that only the Uncaria stem with hooks from Uncaria rhynchophylla, the most widely used in the herbal market, showed the presence of these nine alkaloids. The conversion factors were 1.27, 2.32, 0.98, 1.04, 1.00, 1.02, 1.26, 1.33, and 1.25, respectively. The limits of quantitation were lower than 700ng/mL. The total contents of 31 batches of Uncaria stem with hooks were in the range of 0.1-0.6%, except for Uncaria hirsuta Havil and Uncaria sinensis (Oliv.) Havil. The results also showed that the total content of indole alkaloids tended to decrease with an increase in the hook diameter. This showed that the characteristic chromatogram is practical for controlling the quality of traditional Chinese medicines with multiple botanical origins. |
| WOS关键词 | ALKALOIDS ; IDENTIFICATION ; RHYNCHOPHYLLA ; SINGLE |
| 资助项目 | Special Scientific Research in the Chinese Medicine Industry of State Administration of Traditional Chinese Medicine[201307002] ; National Natural Science Foundation of China[81673591] ; National Natural Science Foundation of China[81403097] ; National Science and Technology Major Project for Major Drug Development[2014ZX09304-307-001-007] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000430965800011 |
| 出版者 | GEORG THIEME VERLAG KG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279821]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Wanying; Guo, De-an |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Haike Rd 501, Shanghai 201203, Peoples R China; 2.China Pharmaceut Univ, Coll Tradit Chinese Med, Nanjing, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Jinjun,Feng, Ruihong,Zhang, Yibei,et al. Characteristic Chromatogram: A Method of Discriminate and Quantitative Analysis for Quality Evaluation of Uncaria Stem with Hooks[J]. PLANTA MEDICA,2018,84(6-7):449-456. |
| APA | Hou, Jinjun.,Feng, Ruihong.,Zhang, Yibei.,Pan, Huiqin.,Yao, Shuai.,...&Guo, De-an.(2018).Characteristic Chromatogram: A Method of Discriminate and Quantitative Analysis for Quality Evaluation of Uncaria Stem with Hooks.PLANTA MEDICA,84(6-7),449-456. |
| MLA | Hou, Jinjun,et al."Characteristic Chromatogram: A Method of Discriminate and Quantitative Analysis for Quality Evaluation of Uncaria Stem with Hooks".PLANTA MEDICA 84.6-7(2018):449-456. |
入库方式： OAI收割
来源：上海药物研究所
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