Green Quantification Strategy Combined with Chemometric Analysis for Triglycerides in Seeds Used in Traditional Chinese Medicine
文献类型：期刊论文
| 作者 | Hou, Jin-Jun2 ; Guo, Ji-ling1; Cao, Chun-Mei3; Yao, Shuai2; Long, Hua-li2; Cai, Lu-Ying2; Da, Juan2; Wu, Wan-Ying2; Guo, De-An2
|
| 刊名 | PLANTA MEDICA
 |
| 出版日期 | 2018-04 |
| 卷号 | 84期号:6-7页码:457-464 |
| 关键词 | triglycerides single standard to determine multicomponents Coix lacryma-jobi var. ma-yuen Poaceae triolein core-shell chemometrics |
| ISSN号 | 0032-0943 |
| DOI | 10.1055/s-0044-100723 |
| 文献子类 | Article |
| 英文摘要 | Triglycerides are the primary constituents of some seed kernels used in traditional Chinese medicine. Quality control of seed kernels containing multiple components with an environmentally friendly method is indispensable for establishing their quality standards (called monographs) in pharmacopeia. Using coix seeds (Semen Coicis) as an example, a green quantification strategy was proposed by combining C-8 core-shell particles with single standard to determine multicomponent technologies to quantify seven triglycerides simultaneously. A core-shell column, namely, Halo C-8 (3.0x100 mm, 2.7 mu m), was used. Methanol was used as the mobile phase at a flow rate of 0.3mL/min, enabling UV detection of the elutes. Seven triglycerides were well separated in 20min, and simultaneously quantified using triolein as a single standard. The conversion factor for each standard was set as 1.0 on ELSD, while for the conversion factors at 203nm, the values increased with the reduction of linoleate. The recovery values were all in the range of 97-107% (RSD <3.0%). The RSD values of precision, including intraday and intermediate precision, were <3.0% when the total content of triglycerides was calculated. The linearity reached r0.9990, and the limit of quantitation reached 40-70ng. Forty-nine batches of coix seeds from four different places of origins and eight batches of adulterants were evaluated and differentiated using principal component analysis. In addition, the validated method was used successfully to quantity seven triglycerides in Semen Persicae, Semen Armeniacae Amarum, and Semen Pruni. |
| WOS关键词 | NF-KAPPA-B ; MULTI-COMPONENTS ; SINGLE MARKER ; CHROMATOGRAPHY ; TRILINOLEIN ; MS |
| 资助项目 | National Natural Science Foundation of China[81403097] ; Special Scientific Research in the Chinese Medicine Industry of State Administration of Traditional Chinese Medicine[201307002] ; National Science and Technology Major Project for Major Drug Development[2014ZX09304-307-001-007] ; Study on International Standardization of Traditional Chinese Medicine[GZYYGJ2017024] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000430965800012 |
| 出版者 | GEORG THIEME VERLAG KG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279822]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Wan-Ying; Guo, De-An |
| 作者单位 | 1.Changchun Univ Chinese Med, Sch Pharmaceut Sci, Changchun, Jilin, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Haike Rd 501, Shanghai 201203, Peoples R China; 3.Univ Sci & Technol China, Nano Sci & Technol Inst, Suzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Jin-Jun,Guo, Ji-ling,Cao, Chun-Mei,et al. Green Quantification Strategy Combined with Chemometric Analysis for Triglycerides in Seeds Used in Traditional Chinese Medicine[J]. PLANTA MEDICA,2018,84(6-7):457-464. |
| APA | Hou, Jin-Jun.,Guo, Ji-ling.,Cao, Chun-Mei.,Yao, Shuai.,Long, Hua-li.,...&Guo, De-An.(2018).Green Quantification Strategy Combined with Chemometric Analysis for Triglycerides in Seeds Used in Traditional Chinese Medicine.PLANTA MEDICA,84(6-7),457-464. |
| MLA | Hou, Jin-Jun,et al."Green Quantification Strategy Combined with Chemometric Analysis for Triglycerides in Seeds Used in Traditional Chinese Medicine".PLANTA MEDICA 84.6-7(2018):457-464. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。