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A sensitive HPLC-MS/MS method for the simultaneous determination of anemoside B4, anemoside A3 and 23-hydroxybetulinic acid: Application to the pharmacokinetics and liver distribution of Pulsatilla chinensis saponins

文献类型：期刊论文

作者Guo, Xiaozhen1,3; Xie, Yang1,2; Lian, Shan1,2; Li, Zhixiong1,3; Gao, Yu1,3; Xu, Zhou1,3; Hu, Pei1,3; Chen, Mingcang1,3; Sun, Zhaolin1,3; Tian, Xiaoting1,3
刊名BIOMEDICAL CHROMATOGRAPHY
出版日期2018-03
卷号32期号:3
关键词23-hydroxybetulinic acid HPLC-MS MS liver distribution pharmacokinetics Pulsatilla chinensis saponins
ISSN号0269-3879
DOI10.1002/bmc.4124
文献子类Article
英文摘要Pulsatilla chinensis saponins, the major active components in the herb, have drawn great attention as potential hepatitis B virus infection and hepatoma treatments. Here, a sensitive and accurate HPLC-MS/MS method was established for simultaneous determination of three saponins - anemoside B4, anemoside A3 and 23-hydroxybetulinic acid - in rat plasma and liver, and fully validated. The method was successfully applied to a pharmacokinetics and liver distribution study of P. chinensis saponins. Consequently, 23-hydroxybetulinic acid, with an extremely low content in the P. chinensis saponins, exhibited the highest exposure in the liver and in sites before and after hepatic disposition, namely, in the portal vein plasma and systemic plasma, followed by anemoside B4, which showed the highest content in the herb, whereas anemoside A3 displayed quite limited exposure. The hepatic first-pass effects were 71% for 23-hydroxybetulinic acid, 27% for anemoside B4 and 37% for anemoside A3, corresponding to their different extents of liver distribution. To our knowledge, this is the first investigation on the liver first-pass effect and distribution of P. chinensis saponins to date. These results also provide valuable information for the understanding of the pharmacological effect of P. chinensis saponins on liver diseases.
WOS关键词MICROVASCULAR ENDOTHELIAL-CELLS ; INGREDIENTS INHIBIT SECRETION ; TRITERPENOIDAL SAPONINS ; LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; BUNGE REGEL ; HEPATITIS-B ; RAT ; APOPTOSIS ; ROOTS
资助项目Shanghai Science and Technology Foundation[14401900700] ; National Natural Science Foundation of China[81374052] ; National Natural Science Foundation of China for Young Scientists[81603280] ; National Natural Science Foundation of China for Young Scientists[81602998] ; China Postdoctoral Science Foundation[8160141707]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000424220400023
出版者WILEY
源URL[http://119.78.100.183/handle/2S10ELR8/279883]  
专题上海中药现代化研究中心
通讯作者Tian, Xiaoting; Huang, Chenggang
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
2.Harbin Univ Commerce, Harbin, Heilongjiang, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China;
推荐引用方式
GB/T 7714		 Guo, Xiaozhen,Xie, Yang,Lian, Shan,et al. A sensitive HPLC-MS/MS method for the simultaneous determination of anemoside B4, anemoside A3 and 23-hydroxybetulinic acid: Application to the pharmacokinetics and liver distribution of Pulsatilla chinensis saponins[J]. BIOMEDICAL CHROMATOGRAPHY,2018,32(3).
APA Guo, Xiaozhen.,Xie, Yang.,Lian, Shan.,Li, Zhixiong.,Gao, Yu.,...&Huang, Chenggang.(2018).A sensitive HPLC-MS/MS method for the simultaneous determination of anemoside B4, anemoside A3 and 23-hydroxybetulinic acid: Application to the pharmacokinetics and liver distribution of Pulsatilla chinensis saponins.BIOMEDICAL CHROMATOGRAPHY,32(3).
MLA Guo, Xiaozhen,et al."A sensitive HPLC-MS/MS method for the simultaneous determination of anemoside B4, anemoside A3 and 23-hydroxybetulinic acid: Application to the pharmacokinetics and liver distribution of Pulsatilla chinensis saponins".BIOMEDICAL CHROMATOGRAPHY 32.3(2018).

入库方式： OAI收割

来源：上海药物研究所

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