Chemical cocktails enable hepatic reprogramming of human urine-derived cells with a single transcription factor
文献类型：期刊论文
| 作者 | Tang, Wei3 ; Guo, Ren2; Shen, Shi-Jun3; Zheng, Yang2; Lu, Yu-Ting1; Jiang, Meng-Meng2; Cui, Xue3; Jiang, Ci-Zhong3; Xie, Xin3
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| 刊名 | Acta pharmacologica Sinica
 |
| 出版日期 | 2018-10-12 |
| ISSN号 | 1745-7254 |
| DOI | 10.1038/s41401-018-0170-z |
| 文献子类 | Article |
| 英文摘要 | Human liver or hepatocyte transplantation is limited by a severe shortage of donor organs. Direct reprogramming of other adult cells into hepatic cells may offer a solution to this problem. In a previous study, we have generated hepatocyte-like cells from mouse fibroblasts using only one transcription factor (TF) plus a chemical cocktail. Here, we show that human urine-derived epithelial-like cells (hUCs) can also be transdifferentiated into human hepatocyte-like cells (hiHeps) using one TF (Foxa3, Hnf1α, or Hnf4α) plus the same chemical cocktail CRVPTD (C, CHIR99021; R, RepSox; V, VPA; P, Parnate; T, TTNPB; and D, Dznep). These hiHeps express multiple hepatocyte-specific genes and display functions characteristic of mature hepatocytes. With the introduction of the large T antigen, these hiHeps can be expanded in vitro and can restore liver function in mice with concanavalin-A-induced acute liver failure. Our study provides a strategy to generate functional hepatocyte-like cells from hUCs by using a single TF plus a chemical cocktail. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266768]  |
| 专题 | 新药研究国家重点实验室 国家新药筛选中心 |
| 通讯作者 | Xie, Xin |
| 作者单位 | 1.University of Chinese Academy of Sciences, Beijing, 100049, China 2.CAS Key Laboratory of Receptor Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; 3.Shanghai Key Laboratory of Signaling and Disease Research, Laboratory of Receptor-Based Biomedicine, School of Life Sciences and Technology, Tongji University, Shanghai, 200092, China; |
| 推荐引用方式 GB/T 7714 | Tang, Wei,Guo, Ren,Shen, Shi-Jun,et al. Chemical cocktails enable hepatic reprogramming of human urine-derived cells with a single transcription factor[J]. Acta pharmacologica Sinica,2018. |
| APA | Tang, Wei.,Guo, Ren.,Shen, Shi-Jun.,Zheng, Yang.,Lu, Yu-Ting.,...&Xie, Xin.(2018).Chemical cocktails enable hepatic reprogramming of human urine-derived cells with a single transcription factor.Acta pharmacologica Sinica. |
| MLA | Tang, Wei,et al."Chemical cocktails enable hepatic reprogramming of human urine-derived cells with a single transcription factor".Acta pharmacologica Sinica (2018). |
入库方式： OAI收割
来源：上海药物研究所
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