Determination of Timosaponin B2 in K-H Buffer by LC-MS and Pharmacokinetics of Timosaponin B2 in Isolated Perfused Rat Liver Model
文献类型：期刊论文
| 作者 | You Jie2; He Lei1; Chen Mingcang1; Ji Yubin2; Pan Guoyu1
|
| 刊名 | Traditional Chinese Drug Research and Clinical Plarmacology
 |
| 出版日期 | 2013 |
| 卷号 | 24期号:1页码:70-74 |
| 关键词 | Timosaponin B2 LC-MS Methodology Matrix effect Liver perfusion |
| ISSN号 | 1003-9783 |
| 其他题名 | LC-MS法测定离体大鼠肝灌流模型中知母皂苷B2的含量及药动学研究 |
| 文献子类 | Article |
| 英文摘要 | Objective To develop a LC-MS method to determine timosaponin B2 in K-H buffer quickly and effectively, and by using this method to study the metabolism of timosaponin B2 in isolated perfused rat liver model. Methods The isolated SD rat liver was perfused with K-H buffer containing timosaponin B2, and then the perfusate was taken out at different time for the determination of timosaponin B2 concentration by LC-MS method. The chromatographic condition was as follows: ACE C_(18) column(100mm*2.1 mm,1mum), mobile phase consisting of acetonitrile -0.05% formic acid V/V),gradient elution. Results The linear ranges of timosaponin B2 was 0.125~2 mug/mL (r~2=0.999). The intra-day and inter-day coefficient of differentiation was less than 10%, and the coefficient of differentiation for matrix effect was less than 15%,which fit the standard. Conclusion The method is effective, rapid and accurate for the determination of timosaponin B2 in K-H buffer, and the hepatic extraction rate and a liver clearance curve of timosaponin B2 at 1.6 mug/mL have beengot in isolated perfused rat liver. |
| 资助项目 | 国家科技重大专项《重大新药创制》课题[00000000] |
| WOS研究方向 | General & Internal Medicine (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4761606 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267796]  |
| 专题 | 药物安全性评价中心 |
| 通讯作者 | Pan Guoyu |
| 作者单位 | 1.Shanghai Medicine Institute, China Academy of Science, Shanghai 201203, China. 2.Research Center for Life Science and Environmental Science, Harbin Commercial University, Harbin, Heilongjiang 150076, China.; |
| 推荐引用方式 GB/T 7714 | You Jie,He Lei,Chen Mingcang,et al. Determination of Timosaponin B2 in K-H Buffer by LC-MS and Pharmacokinetics of Timosaponin B2 in Isolated Perfused Rat Liver Model[J]. Traditional Chinese Drug Research and Clinical Plarmacology,2013,24(1):70-74. |
| APA | You Jie,He Lei,Chen Mingcang,Ji Yubin,&Pan Guoyu.(2013).Determination of Timosaponin B2 in K-H Buffer by LC-MS and Pharmacokinetics of Timosaponin B2 in Isolated Perfused Rat Liver Model.Traditional Chinese Drug Research and Clinical Plarmacology,24(1),70-74. |
| MLA | You Jie,et al."Determination of Timosaponin B2 in K-H Buffer by LC-MS and Pharmacokinetics of Timosaponin B2 in Isolated Perfused Rat Liver Model".Traditional Chinese Drug Research and Clinical Plarmacology 24.1(2013):70-74. |
入库方式： OAI收割
来源：上海药物研究所
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