Variability of directed differentiation potential into neurons between neural stem cells from embryonic rats and immortalized neural stem cell line C17.2
文献类型：期刊论文
| 作者 | Xie Junling2; Zhang Yue2; Huang Xiaojie2; Zang Yi1
|
| 刊名 | Acta Anatomica Sinica
 |
| 出版日期 | 2012 |
| 卷号 | 43期号:5页码:581-587 |
| 关键词 | Neural stem cell Neuron Cell differentiation Retinoic acid Nestin betaⅢ tubulin Immunohistochemistry Rat |
| ISSN号 | 0529-1356 |
| 其他题名 | 大鼠胚胎神经干细胞与永生化神经干细胞系C17.2定向分化为神经元潜能的差异 |
| 文献子类 | Article |
| 英文摘要 | Objective To compare directed differentiation potential into neurons between neural stem cells(NSCs) from early embryonic rats and immortalized neural stem cell line C17.2(C17.2-NSC), and to establish a screening assay on the level of NSCs for high content screening in order to discover drugs that induce directed neurons differentiation of NSCs. Methods Three types of cells, the C17.2-NSC, 17 days embryonic hippocampus NSCs and 11 days embryonic cerebral cortex NSCs were used in this study. Cells from each type were divided into the control and experimental groups, and induced to differentiate by 0 mumol/L and 1 mumol/L retinoic acid (RA) for 5 days under DMEM/F12 medium containing 2% (V/V) B27 and 37℃, 5% C02 normal culture condition. The variability of expression level of nestin and beta Ⅲ tubulin (Tujl), which are the specific markers of neural stem and progenitor cells and neurons, respectively, in both control group and experimental group were detected through immunohistochemistry. Results Compared with the control group, C17.2-NSC did not differentiate into neurons under the induction of 1 mumol/L RA, but 17-day embryonic hippocampus NSCs and 11-day embryonic cerebral cortex NSCs effectively differentiated into neurons under the same treatment. Conclusion The directed differentiation capacity of early embryonic NSCs into neurons is higher than that of C17.2-NSC, thus early embryonic NSCs are suitable for high content drug screening which aims to discover new types of compounds that induce directed neurons differentiation of NSCs. |
| 资助项目 | 国家自然科学基金资助项目[00000000] |
| WOS研究方向 | General & Internal Medicine (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4696544 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267813]  |
| 专题 | 国家新药筛选中心 |
| 作者单位 | 1.National Center for Drug Screening, Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China. 2.School of Life Sciences, East China Normal University, Shanghai 200062, China.; |
| 推荐引用方式 GB/T 7714 | Xie Junling,Zhang Yue,Huang Xiaojie,et al. Variability of directed differentiation potential into neurons between neural stem cells from embryonic rats and immortalized neural stem cell line C17.2[J]. Acta Anatomica Sinica,2012,43(5):581-587. |
| APA | Xie Junling,Zhang Yue,Huang Xiaojie,&Zang Yi.(2012).Variability of directed differentiation potential into neurons between neural stem cells from embryonic rats and immortalized neural stem cell line C17.2.Acta Anatomica Sinica,43(5),581-587. |
| MLA | Xie Junling,et al."Variability of directed differentiation potential into neurons between neural stem cells from embryonic rats and immortalized neural stem cell line C17.2".Acta Anatomica Sinica 43.5(2012):581-587. |
入库方式： OAI收割
来源：上海药物研究所
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