Construction of Recombinant Adenovirus Expression Vector of Dominant Negative AMPK and Its Evaluation in Mouse Myoblast Cell Line C2C12 Differentiation into Myotube
文献类型：期刊论文
| 作者 | Yang Chenghong2; Deng Sisi1; Zang Yi1
|
| 刊名 | Biotechnology Bulletin
 |
| 出版日期 | 2012 |
| 期号 | 4页码:135-140 |
| 关键词 | DN-AMPK C2C12 Dominant negative-AMPK Adenovirus C2C12 Myotube differentiation |
| ISSN号 | 1002-5464 |
| 其他题名 | DN-AMPK腺病毒载体的构建及其在小鼠成肌细胞系C2C12肌管分化中的影响 |
| 文献子类 | Article |
| 英文摘要 | In order to evaluate the potency of AMPK,usualy we use dominant negative AMPK(DN-AMPK)as a tool.For this reason,we constructed recombinant adenovirus expression vector of DN-AMPK(AMPKalpha1(D159A)and AMPKalpha2(K45R)),which were transfected into mouse myoblast cell line C2C12 to detect the regulation of DN-AMPK during the C2C12 myotube differentiation.The recombinant plasmid was packaged and amplified after being transfected into HEK293 cells.After infecting C2C12 myoblast with recombinant adenovirus vector,the adenoviral infection was determined by iCys Research Imaging Cytometer.The infection efficiency was almost 100% for 6 days and Western blot indicated that the expression of AMPK in C2C12 of recombinant adenoviral infection group was signifcantly higher than the control group.At the same time,when DN-AMPK was overexpressed,SN-5(AMPK activator)could not induce activation of ACC.During the differentiation of C2C12 myoblast,DN-AMPK can increase the expression of Myod and Myogenin(myotube marker).These results indicated that the overexpression of DN-AMPK could increase the level of differentiation of C2C12 into myotube. |
| 资助项目 | 国家重大科学研究计划蛋白质专项[00000000] ; 上海市公共服务平台项目[00000000] |
| WOS研究方向 | Genetics & Heredity (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4499496 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267860]  |
| 专题 | 国家新药筛选中心 |
| 通讯作者 | Zang Yi |
| 作者单位 | 1.National Center for Drug Screening,Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China. 2.School of Life Science,East China Normal University, Shanghai 200062, China.; |
| 推荐引用方式 GB/T 7714 | Yang Chenghong,Deng Sisi,Zang Yi. Construction of Recombinant Adenovirus Expression Vector of Dominant Negative AMPK and Its Evaluation in Mouse Myoblast Cell Line C2C12 Differentiation into Myotube[J]. Biotechnology Bulletin,2012(4):135-140. |
| APA | Yang Chenghong,Deng Sisi,&Zang Yi.(2012).Construction of Recombinant Adenovirus Expression Vector of Dominant Negative AMPK and Its Evaluation in Mouse Myoblast Cell Line C2C12 Differentiation into Myotube.Biotechnology Bulletin(4),135-140. |
| MLA | Yang Chenghong,et al."Construction of Recombinant Adenovirus Expression Vector of Dominant Negative AMPK and Its Evaluation in Mouse Myoblast Cell Line C2C12 Differentiation into Myotube".Biotechnology Bulletin .4(2012):135-140. |
入库方式： OAI收割
来源：上海药物研究所
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