Quality control of Tremella fuciformis polysaccharide by HPGPC
文献类型：期刊论文
| 作者 | LIU Qin2; NING Jialing3; DING Kan2
|
| 刊名 | Chinese Traditional and Herbal Drugs
 |
| 出版日期 | 2011 |
| 卷号 | 42期号:9页码:1732-1735 |
| 关键词 | Tremella fuciformis Berk. Tremella fuciformis polysaccharide high performance gel permeation chromatography(HPGPC) quality control polysaccharide molecular weight |
| ISSN号 | 0253-2670 |
| 其他题名 | 基于高效凝胶渗透色谱法的银耳多糖质量控制研究 |
| 文献子类 | Article |
| 英文摘要 | Objective To develop a high performance gel permeation chromatography(HPGPC)method for quality control of Tremella fuciformis polysaccharide.Methods The separations were carried out with two series-wound columns including Shodex Sugar KS-804(300 mm*8 mm,7mum)and KS-805(300 mm*8 mm,17mum),water as mobile phase at flow rate 0.8 mL/min,column temperature at 40℃,injection volume 40muL,and differential index detector.Results T.fuciformis polysaccharide peak was located within 14.4015.30 min and corresponding molecular weight was 1.26*10~6 1.39*10~6.The linearity was good during 0.012.0 mg/mL(r=0.999).The polysaccharide concentration was 1.11.5 mg/mL and 0.81.1 mg/mL with precipitation of T.fuciformis polysaccharide and blue dextran as reference,respectively.LOD and LOQ were 0.8 and 2mug,respectively.Conclusion The method is simple,quick,and accurate,and has good repeatability and high sensitivity,which is fitful for quality control. |
| 资助项目 | 国家自然科学基金资助项目[00000000] |
| WOS研究方向 | General & Internal Medicine (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4312260 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267869]  |
| 专题 | 国家新药筛选中心 |
| 作者单位 | 1.Chinese Academy of Sciences, Shanghai 201203, China.; 2.Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Glycochemistry and Glycobiology Laboratory,Chinese Academy of Sciences; 3.Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Joint Laboratory for the Research of Chinese Herbal Polysaccharides,Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | LIU Qin,NING Jialing,DING Kan. Quality control of Tremella fuciformis polysaccharide by HPGPC[J]. Chinese Traditional and Herbal Drugs,2011,42(9):1732-1735. |
| APA | LIU Qin,NING Jialing,&DING Kan.(2011).Quality control of Tremella fuciformis polysaccharide by HPGPC.Chinese Traditional and Herbal Drugs,42(9),1732-1735. |
| MLA | LIU Qin,et al."Quality control of Tremella fuciformis polysaccharide by HPGPC".Chinese Traditional and Herbal Drugs 42.9(2011):1732-1735. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。