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Expression and function analysis of chemokine receptor CCR6 in HEK293 cells

文献类型：期刊论文

作者Wei Wei2; Gao Lan2; Zhang Feifei1; Cui Lixin1; Xie Xin1
刊名Chinese Journal of Cellular and Molecular Immunology
出版日期2011
卷号27期号:1页码:33
关键词chemokine receptor 6 HEK 293 cell line expression
ISSN号1007-8738
其他题名人趋化因子受体CCR6在HEK293细胞内的稳定表达及其功能分析
文献子类Article
英文摘要AIM: To construct a cell line which stably expresses human chemokine receptor 6 (CCR6). METHODS: The human CCR6 cDNA and plasmid G were co-transfected into HEK 293 cells and the clones stably expressing CCR6 were picked out. The expression of CCR6 in HEK293 cells was detected by RT-PCR, Western blotting, immunofluorescence test, calcium mobilization and in vitro chemotaxis assay. RESULTS: The transfected HEK293 cells could stably express functional human CCR6. CONCLUSION: Successfully establish a cell line which stably express human CCR6 and lays the foundation for its biological function's study and specific antagonist screening
资助项目科技部新药创制重大专题项目[00000000] ; 上海市公共研发平台项目[00000000] ; 国家自然基金资助项目[00000000]
WOS研究方向Cell Biology (provided by Clarivate Analytics)
语种中文
CSCD记录号CSCD:4123987
源URL[http://119.78.100.183/handle/2S10ELR8/267897]  
专题国家新药筛选中心
作者单位1.The National Center for New Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
2.School of Life Sciences, Lanzhou University, Lanzhou, Gansu 730000, China.;
推荐引用方式
GB/T 7714		 Wei Wei,Gao Lan,Zhang Feifei,et al. Expression and function analysis of chemokine receptor CCR6 in HEK293 cells[J]. Chinese Journal of Cellular and Molecular Immunology,2011,27(1):33.
APA Wei Wei,Gao Lan,Zhang Feifei,Cui Lixin,&Xie Xin.(2011).Expression and function analysis of chemokine receptor CCR6 in HEK293 cells.Chinese Journal of Cellular and Molecular Immunology,27(1),33.
MLA Wei Wei,et al."Expression and function analysis of chemokine receptor CCR6 in HEK293 cells".Chinese Journal of Cellular and Molecular Immunology 27.1(2011):33.

入库方式： OAI收割

来源：上海药物研究所

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