Three-dimensional visualization of fibrochondrocytes in rabbit patella-patellar tendon junction using synchrotron radiation-based X-ray phase contrast tomography
文献类型：期刊论文
| 作者 | Chen Huabin1; Hu Jianzhong2; Zhou Jingyong1; Zheng Cheng1; Wang Zhanwen1; Chen Can1; Cao Yong2; Yin Xianzhen3; Lyu Hongbin1 |
| 刊名 | Nuclear Techniques
 |
| 出版日期 | 2015 |
| 卷号 | 38期号:11页码:110101 |
| 关键词 | Synchrotron radiation Phase contrast imaging Bone-to-tendon junction Fibrochondrocytes Three-dimensional |
| ISSN号 | 0253-3219 |
| 其他题名 | 同步辐射X线相衬成像技术在兔髌骨-髌腱连接点纤维软骨细胞的三维可视化应用研究 |
| 文献子类 | Article |
| 英文摘要 | Background: Synchrotron radiation-based X-ray phase-contrast computed tomography (SR-XPCT) is an advanced tool for nondestructive analysis of three dimensional inner microstructure of samples, and plays an important part in the researches such as spinal cord microvasculature and lung bronchium. Purpose: This study was to investigate the three-dimensional morphology of fibrochondrocytes in rabbit patella-patellar tendon junction (PPTJ) using SR-XPCT, and develop an advanced imaging method. Methods: Three PPTJs were harvested from healthy mature female New Zealand rabbits. The specimens were scanned by SR-XPCT at BL13W1 of Shanghai Synchrotron Radiation Facility (SSRF) in China. The tomographic image was captured by Charge Coupled Device (CCD) detector with a 0.65-mum resolution. The three-dimensional visualization images of PPTJ were reconstructed by VG Studio Max. After that, the specimens were embedded by paraffin for Safranin O/Fast-green staining. Results: High-resolution three-dimensional visualization images of PPTJ and the parameters of the chondrocytes in fibrocartilage zone were successfully obtained via the SR-XPCT. With spherical and ellipsoidal morphologic features and its configuration characteristics in three dimension, the fibrochondrocytes revealed by SR-XPCT were consistent with the results of traditional histological staining. The parameters of chondrocytes showed that the mean diameter of chondrocyte was (10.139 1.265) mum, the mean volume was (291.18787.283) mum~3 and 75.4% of the chondrocytes' volume ranged from 200 mum~3 to 400 mum~3, the mean sphericity degree was 0.711 0.079, and 84.9% of chondrocytes' sphericity degree ranged from 0.605 to 0.805. Conclusion: The three-dimensional microstructure of the PPTJ was intuitively, accurately and undistortedly realized by the SR-XPCT, which may act as a novel instrument to access the three-dimensional microstructure changes during the PPTJ healing process. |
| 资助项目 | 国家自然科学基金[00000000] |
| WOS研究方向 | Nuclear Science & Technology (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5566383 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269266]  |
| 专题 | 分子影像中心（筹） |
| 作者单位 | 1.Department of Sports Medicine and Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; 2.Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.; 3.Center for Drug Delivery System, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | Chen Huabin,Hu Jianzhong,Zhou Jingyong,et al. Three-dimensional visualization of fibrochondrocytes in rabbit patella-patellar tendon junction using synchrotron radiation-based X-ray phase contrast tomography[J]. Nuclear Techniques,2015,38(11):110101. |
| APA | Chen Huabin.,Hu Jianzhong.,Zhou Jingyong.,Zheng Cheng.,Wang Zhanwen.,...&Lyu Hongbin.(2015).Three-dimensional visualization of fibrochondrocytes in rabbit patella-patellar tendon junction using synchrotron radiation-based X-ray phase contrast tomography.Nuclear Techniques,38(11),110101. |
| MLA | Chen Huabin,et al."Three-dimensional visualization of fibrochondrocytes in rabbit patella-patellar tendon junction using synchrotron radiation-based X-ray phase contrast tomography".Nuclear Techniques 38.11(2015):110101. |
入库方式： OAI收割
来源：上海药物研究所
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