Chemical Cocktails Enable Hepatic Reprogramming of Mouse Fibroblasts with a Single Transcription Factor
文献类型：期刊论文
| 作者 | Guo, Ren2,4; Tang, Wei3 ; Yuan, Qianting2; Hui, Lijian5; Wang, Xin1,6; Xie, Xin2,3
|
| 刊名 | STEM CELL REPORTS
 |
| 出版日期 | 2017-08-08 |
| 卷号 | 9期号:2页码:499-512 |
| ISSN号 | 2213-6711 |
| DOI | 10.1016/j.stemcr.2017.06.013 |
| 文献子类 | Article |
| 英文摘要 | Liver or hepatocytes transplantation is limited by the availability of donor organs. Functional hepatocytes independent of the donor sources may have wide applications in regenerative medicine and the drug industry. Recent studies have demonstrated that chemical cocktails may induce reprogramming of fibroblasts into a range of functional somatic cells. Here, we show that mouse fibroblasts can be transdifferentiated into the hepatocyte-like cells (iHeps) using only one transcription factor (TF) (Foxa1, Foxa2, or Foxa3) plus a chemical cocktail. These iHeps show typical epithelial morphology, express multiple hepatocyte-specific genes, and acquire hepatocyte functions. Genetic lineage tracing confirms the fibroblast origin of these iHeps. More interestingly, these iHeps are expandable in vitro and can reconstitute the damaged hepatic tissues of the fumarylacetoacetate hydrolase-deficient (Fah (-)/(-)) mice. Our study provides a strategy to generate functional hepatocyte-like cells by using a single TF plus a chemical cocktail and is one step closer to generate the full-chemical iHeps. |
| WOS关键词 | PLURIPOTENT STEM-CELLS ; NUCLEAR FACTOR 4-ALPHA ; LIVER DEVELOPMENT ; FUNCTIONAL HEPATOCYTES ; DEFINED FACTORS ; EXPRESSION ; DRUG ; DIFFERENTIATION ; METABOLISM ; GENERATION |
| 资助项目 | Ministry of Science and Technology of China[2015CB964503] ; Chinese Academy of Sciences[XDA01040301] ; National Natural Science Foundation of China[81425024] ; National Natural Science Foundation of China[31371511] ; National Natural Science Foundation of China[81472862] ; National Natural Science Foundation of China[31501189] ; Shanghai Science and Technology Committee[14ZR1409500] ; Shanghai Science and Technology Committee[15YF1402400] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000407105000009 |
| 出版者 | CELL PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272531]  |
| 专题 | 国家新药筛选中心 |
| 通讯作者 | Xie, Xin |
| 作者单位 | 1.Inner Mongolia Univ, Minist Mammalian Reprod Biol & Biotechnol, Key Lab Natl Educ, Hohhot 010021, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, CAS Key Lab Receptor Res, 189 Guo Shou Jing Rd, Shanghai 201203, Peoples R China; 3.Tongji Univ, Sch Life Sci & Technol, Lab Receptor Based Biomed, Shanghai Key Lab Signaling & Dis Res, Shanghai 200092, Peoples R China; 4.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China; 6.Univ Minnesota, Stem Cell Inst, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA |
| 推荐引用方式 GB/T 7714 | Guo, Ren,Tang, Wei,Yuan, Qianting,et al. Chemical Cocktails Enable Hepatic Reprogramming of Mouse Fibroblasts with a Single Transcription Factor[J]. STEM CELL REPORTS,2017,9(2):499-512. |
| APA | Guo, Ren,Tang, Wei,Yuan, Qianting,Hui, Lijian,Wang, Xin,&Xie, Xin.(2017).Chemical Cocktails Enable Hepatic Reprogramming of Mouse Fibroblasts with a Single Transcription Factor.STEM CELL REPORTS,9(2),499-512. |
| MLA | Guo, Ren,et al."Chemical Cocktails Enable Hepatic Reprogramming of Mouse Fibroblasts with a Single Transcription Factor".STEM CELL REPORTS 9.2(2017):499-512. |
入库方式： OAI收割
来源：上海药物研究所
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