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One-step generation of mice carrying a conditional allele together with an HA-tag insertion for the delta opioid receptor

文献类型：期刊论文

作者Su, Dongru1; Wang, Min2; Ye, Chenli1; Fang, Jiahui1; Duan, Yanhui1; Zhang, Zhenghong1; Hua, Qiuhong1; Shi, Changjie1; Zhang, Lihong1; Zhang, Ru1
刊名SCIENTIFIC REPORTS
出版日期2017-03-16
卷号7
ISSN号2045-2322
DOI10.1038/srep44476
文献子类Article
英文摘要G protein-coupled receptors (GPCRs) are important modulators of many physiological functions and excellent drug targets for many diseases. However, to study the functions of endogenous GPCRs is still a challenging task, partially due to the low expression level of GPCRs and the lack of highly potent and selective GPCR antibodies. Overexpression or knock-in of tagged GPCRs, or knockout of specific GPCRs in mice, are common strategies used to study the in vivo functions of these receptors. However, generating separate mice carrying tagged GPCRs or conditional alleles for GPCRs is labor intensive, and requires additional breeding costs. Here we report the generation of mice carrying an HA-tagged DOR (delta opioid receptor) flanked by LoxP sequences at the endogenous DOR locus using a single recombination step, aided by the TALEN system. These animals can be used directly to study the expression, localization, protein-protein interaction and signal transduction of endogenous DOR using anti-HA antibodies. By crossing with mice expressing tissue-specific Cre, these mice can also generate offspring with DOR knockout within specific tissues. These mice are powerful tools to study the in vivo functions of DOR. Furthermore, the gene modification strategy could also be used to study the functions of many other GPCRs.
WOS关键词PROTEIN-COUPLED RECEPTORS ; MUSCARINIC ACETYLCHOLINE-RECEPTORS ; BETA-ARRESTIN ; IN-VIVO ; EXPRESSION ; NEURONS ; MOUSE ; ANTIBODIES ; GPR3 ; MU
资助项目Ministry of Science and Technology of China[2015CB964503] ; Ministry of Science and Technology of China[2014CB965002] ; Ministry of Science and Technology of China[2013ZX09507001] ; Ministry of Science and Technology of China[2014CB541906] ; Ministry of Science and Technology of China[2011CB965104] ; National Natural Science Foundation of China[81425024] ; National Natural Science Foundation of China[31371511] ; Shanghai Commission of Science and Technology[15JC1400202] ; Shanghai Commission of Science and Technology[15DZ2291600]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000396539400001
出版者NATURE PUBLISHING GROUP
源URL[http://119.78.100.183/handle/2S10ELR8/272740]  
专题国家新药筛选中心
通讯作者Zhang, Ru; Xie, Xin
作者单位1.Tongji Univ, Collaborat Innovat Ctr Brain Sci, Sch Life Sci & Technol, Shanghai Key Lab Signaling & Dis Res,Lab Receptor, Shanghai 200092, Peoples R China;
2.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Su, Dongru,Wang, Min,Ye, Chenli,et al. One-step generation of mice carrying a conditional allele together with an HA-tag insertion for the delta opioid receptor[J]. SCIENTIFIC REPORTS,2017,7.
APA Su, Dongru.,Wang, Min.,Ye, Chenli.,Fang, Jiahui.,Duan, Yanhui.,...&Xie, Xin.(2017).One-step generation of mice carrying a conditional allele together with an HA-tag insertion for the delta opioid receptor.SCIENTIFIC REPORTS,7.
MLA Su, Dongru,et al."One-step generation of mice carrying a conditional allele together with an HA-tag insertion for the delta opioid receptor".SCIENTIFIC REPORTS 7(2017).

入库方式： OAI收割

来源：上海药物研究所

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