AICAR induces astroglial differentiation of neural stem cells via activating the JAK/STAT3 pathway independently of AMP-activated protein kinase
文献类型：期刊论文
| 作者 | Zang, Yi1 ; Yu, Li-Fang1; Pang, Tao1; Fang, Lei-Ping3; Feng, Xu1; Wen, Tie-Qiao2; Nan, Fa-Jun1; Feng, Lin-Yin3; Li, Jia1
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| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
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| 出版日期 | 2008-03-07 |
| 卷号 | 283期号:10页码:6201-6208 |
| ISSN号 | 0021-9258 |
| DOI | 10.1074/jbc.M708619200 |
| 文献子类 | Article |
| 英文摘要 | Neural stem cell differentiation and the determination of lineage decision between neuronal and glial fates have important implications in the study of developmental, pathological, and regenerative processes. Although small molecule chemicals with the ability to control neural stem cell fate are considered extremely useful tools in this field, few were reported. AICAR is an adenosine analog and extensively used to activate AMP-activated protein kinase (AMPK), a metabolic "fuel gauge" of the biological system. In the present study, we found an unrecognized astrogliogenic activity of AICAR on not only immortalized neural stem cell line C17.2 (C17.2-NSC), but also primary neural stem cells (NSCs) derived from post-natal (P0) rat hippocampus (P0-NSC) and embryonic day 14 (E14) rat embryonic cortex (E14-NSC). However, another AMPK activator, Metformin, did not alter either the C17.2-NSC or E14-NSC undifferentiated state although both Metformin and AICAR can activate the AMPK pathway in NSC. Furthermore, overexpression of dominant-negative mutants of AMPK in C17.2-NSC was unable to block the gliogenic effects of AICAR. We also found AICAR could activate the Janus kinase (JAK) STAT3 pathway in both C17.2-NSC and E14-NSC but Metformin fails. JAK inhibitor I abolished the gliogenic effects of AICAR. Taken together, these results suggest that the astroglial differentiation effect of AICAR on neural stem cells was acting independently of AMPK and that the JAK-STAT3 pathway is essential for the gliogenic effect of AICAR. |
| WOS关键词 | CENTRAL-NERVOUS-SYSTEM ; LEUKEMIA INHIBITORY FACTOR ; IN-VIVO ; 5-AMINO-4-IMIDAZOLECARBOXAMIDE RIBOSIDE ; ASTROCYTE DIFFERENTIATION ; NEURONAL SURVIVAL ; MOUSE-BRAIN ; FATTY-ACID ; PROGENITOR ; ASTROGLIOGENESIS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000253779500027 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272960]  |
| 专题 | 国家新药筛选中心 药物安全性评价中心 |
| 通讯作者 | Li, Jia |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China; 2.Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Neurol Pharmacol Dept, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zang, Yi,Yu, Li-Fang,Pang, Tao,et al. AICAR induces astroglial differentiation of neural stem cells via activating the JAK/STAT3 pathway independently of AMP-activated protein kinase[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2008,283(10):6201-6208. |
| APA | Zang, Yi.,Yu, Li-Fang.,Pang, Tao.,Fang, Lei-Ping.,Feng, Xu.,...&Li, Jia.(2008).AICAR induces astroglial differentiation of neural stem cells via activating the JAK/STAT3 pathway independently of AMP-activated protein kinase.JOURNAL OF BIOLOGICAL CHEMISTRY,283(10),6201-6208. |
| MLA | Zang, Yi,et al."AICAR induces astroglial differentiation of neural stem cells via activating the JAK/STAT3 pathway independently of AMP-activated protein kinase".JOURNAL OF BIOLOGICAL CHEMISTRY 283.10(2008):6201-6208. |
入库方式： OAI收割
来源：上海药物研究所
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