Berberine-stimulated glucose uptake in L6 myotubes involves both AMPK and p38 MAPK
文献类型：期刊论文
| 作者 | Cheng, Zhe; Pang, Tao; Gu, Min ; Gao, An-Hui; Xie, Chuan-Ming; Li, Jing-Ya; Nan, Fa-Jun; Li, Jia
|
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
 |
| 出版日期 | 2006-11 |
| 卷号 | 1760期号:11页码:1682-1689 |
| 关键词 | berberine glucose uptake AMPK p38 MAPK myotube |
| ISSN号 | 0304-4165 |
| DOI | 10.1016/j.bbagen.2006.09.007 |
| 文献子类 | Article |
| 英文摘要 | Berberine is a plant alkaloid used in traditional Chinese medicine and has been reported to have antihyperglycemic activity in NIDDM patients. However, the molecular basis for this action is yet to be elucidated. Here we investigate the effects and signaling pathways of berberine on L6 rat skeletal muscles. Our study demonstrates that berberine stimulates glucose uptake in a time- and dose-dependent manner. Intriguingly, berberine-stimulated glucose uptake does not vary as insulin concentration increases, and could not be blocked by the PI 3-kinase inhibitor wortmannin. Berberine only weakly stimulates the phosphorylation of Akt/PKB, a key molecule in the insulin signaling pathway, but strongly promotes the phosphorylation of AMPK and p38 MAPK. The effects of berberine are not a result of pro-oxidant action, but a consequence of an increased cellular AMP:ATP ratio. Moreover, berberine-stimulated glucose uptake is inhibited by the AMPK inhibitor Compound C and the p38 MAPK inhibitor SB202190. Inhibition of AMPK reduces p38 MAPK phosphorylation, suggesting that AMPK lies upstream of p38 MAPK. These results suggest that berberine circumvents insulin signaling pathways and stimulates glucose uptake through the AMP-AMPK-p38 MAPK pathway, which may account for the antilryperglycemic effects of this drug. (c) 2006 Elsevier B.V. All rights reserved. |
| WOS关键词 | ACTIVATED PROTEIN-KINASE ; SKELETAL-MUSCLE ; GLUT4 TRANSLOCATION ; SIGNALING PATHWAYS ; MAMMALIAN TARGET ; POTENTIAL ROLE ; DNA-SYNTHESIS ; IN-VITRO ; TRANSPORT ; INSULIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000242669200013 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273452]  |
| 专题 | 国家新药筛选中心 药物安全性评价中心 |
| 通讯作者 | Li, Jia |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Zhe,Pang, Tao,Gu, Min,et al. Berberine-stimulated glucose uptake in L6 myotubes involves both AMPK and p38 MAPK[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2006,1760(11):1682-1689. |
| APA | Cheng, Zhe.,Pang, Tao.,Gu, Min.,Gao, An-Hui.,Xie, Chuan-Ming.,...&Li, Jia.(2006).Berberine-stimulated glucose uptake in L6 myotubes involves both AMPK and p38 MAPK.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1760(11),1682-1689. |
| MLA | Cheng, Zhe,et al."Berberine-stimulated glucose uptake in L6 myotubes involves both AMPK and p38 MAPK".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1760.11(2006):1682-1689. |
入库方式： OAI收割
来源：上海药物研究所
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