Cloning, expression and characterization of human vascular endothelial growth factor receptor 1 tyrosine kinase
文献类型：期刊论文
| 作者 | Zhuang, SF; Ye, QZ |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002-01 |
| 卷号 | 34期号:1页码:39-44 |
| 关键词 | vascular endothelial growth factor receptor tyrosine kinase fusion protein |
| ISSN号 | 0582-9879 |
| 文献子类 | Article |
| 英文摘要 | Vascular endothelial growth factor receptor 1 (Flt1) plays an important role in angiogenesis. It was hypothesized that, upon binding to VEGF, Flt1 tyrosine kinase underwent dimerization and initiated the signal transduction in VEGF/VEGF receptor system. In this report, a soluble active Flt1 tyrosine kinase domain expressed in E. coli was obtained, and its properties were partly characterized. The eDNA of Flt1 tyrosine kinase domain was obtained from the total RNA extracted from human liver cancer tissues by using RT-PCR, and was cloned to vector pGEX-KG. A soluble active GST-fusion protein of Flt1 tyrosine kinase domain (GST-F) was obtained from E. coli BL21 (DE3) pLysS. Athough it was reported that GST-F contains no phosphorylation site, it did autophospliorylate in vitro. Mg2+ and Mn2+ were essential for the activity. It was also found that GST-F phosphorylated a synthesized substrate PolyE4Y, but nut MBP and Sre-related-peptide. The optimal Mg2+ and Mn2+ concentration for polyE4Y phosphorylation was 15 mmol/L and 0.5 mmol/L, respectively. This work is helpful for developing the new anti-cancer drugs. |
| WOS关键词 | PHOSPHORYLATION SITES ; VEGF-RECEPTOR ; SH2 DOMAIN ; BINDING ; FLT-1 ; IDENTIFICATION ; PROTEINS ; KDR |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 中文 |
| CSCD记录号 | CSCD:1140939 |
| WOS记录号 | WOS:000173426700008 |
| 出版者 | SHANGHAI INST BIOCHEMISTRY, ACADEMIA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274405]  |
| 专题 | 国家新药筛选中心 |
| 作者单位 | Chinese Acad Sci, Natl Ctr Drug Screening, Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhuang, SF,Ye, QZ. Cloning, expression and characterization of human vascular endothelial growth factor receptor 1 tyrosine kinase[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(1):39-44. |
| APA | Zhuang, SF,&Ye, QZ.(2002).Cloning, expression and characterization of human vascular endothelial growth factor receptor 1 tyrosine kinase.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(1),39-44. |
| MLA | Zhuang, SF,et al."Cloning, expression and characterization of human vascular endothelial growth factor receptor 1 tyrosine kinase".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.1(2002):39-44. |
入库方式： OAI收割
来源：上海药物研究所
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