Development of a K562 cell-based assay for screening anticancer agents
文献类型：期刊论文
| 作者 | Qian, J; Zhou, CH; Qian, Z; Nan, FJ ; Ye, QZ
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2001-09 |
| 卷号 | 22期号:9页码:821-826 |
| 关键词 | antitumor drug screening assays K562 cells cell culture drug screening antineoplastic agents |
| ISSN号 | 1671-4083 |
| 文献子类 | Article |
| 英文摘要 | AIM: To develop a leukemia cell line K562-based assay for high-throughput screening. METHODS : The screening was carried out on 96-well plates with monitoring cell proliferation by a combined 3-[4, 5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl]-2H-tetrazolium (MTS)/phenazine methosulfate (PMS) method. Conditions for evaluating effects on the proliferation of K562 cells by individual compounds on the 96-well plates were optimized. RESULTS: A set of 800 small organic compounds was screened for anticancer activity by this cell-based assay, with consumption of each compound at 500 ng. Eleven compounds were identified with > 80 % inhibitory activity at 5 mg/L, among which 9 compounds were confirmed by subsequent testing at multiple concentrations. The most potent compound showed an IC50 at 170 nmol/L, and there were total of 7 compounds showed IC50 less than 10 mu mol/L. CONCLUSION: The high-throughput method using K562 cell line is fast, economical, effective, and practical in identifying inhibitors as potential therapeutic agents for cancer. |
| WOS关键词 | MTS ; XTT |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:515890 |
| WOS记录号 | WOS:000171146200010 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274448]  |
| 专题 | 国家新药筛选中心 |
| 通讯作者 | Ye, QZ |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qian, J,Zhou, CH,Qian, Z,et al. Development of a K562 cell-based assay for screening anticancer agents[J]. ACTA PHARMACOLOGICA SINICA,2001,22(9):821-826. |
| APA | Qian, J,Zhou, CH,Qian, Z,Nan, FJ,&Ye, QZ.(2001).Development of a K562 cell-based assay for screening anticancer agents.ACTA PHARMACOLOGICA SINICA,22(9),821-826. |
| MLA | Qian, J,et al."Development of a K562 cell-based assay for screening anticancer agents".ACTA PHARMACOLOGICA SINICA 22.9(2001):821-826. |
入库方式： OAI收割
来源：上海药物研究所
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